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Tissue Culture System Screening of Actinidia chinensis cv.Hongyang ‘Sanxia Horg’(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2016年09
Page:
114-117
Research Field:
Publishing date:

Info

Title:
Tissue Culture System Screening of Actinidia chinensis cv.Hongyang ‘Sanxia Horg’
Author(s):
GAN LipingRUAN ShenqingZENG Xiaolin
(College of Life Science and Engineering,Chongqing Three Gorges University,Chongqing 404100)
Keywords:
Actinidia chinensis cv.Hongyangtissue culturecallus
PACS:
-
DOI:
10.11937/bfyy.201609030
Abstract:
Taking the leaf,stems,buds of Hongyang kiwi fruit as explants,MS as basic medium,the effect of different disinfection methods,callus and bud induction ability of hormone formulations was studied.The results showed that the disinfectant method of 75% alcohol treatment explants 30 s,then 1% NaClO disinfection 10 min was the best with sterilizing effect reached 75.0%;callus optimum medium was MS+1.0 mg/L ZT+0.1 mg/L NAA while optimum medium for inducing adventitious buds was MS+1.0 mg/L 6-BA+0.1 mg/L NAA.The stems was the best explants for callus formation.

References:

 

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Last Update: 2016-07-07