|Table of Contents|

Establishment of Regeneration System of ‘Xinyu’ Grapevine in vitro(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2016年09
Page:
109-113
Research Field:
Publishing date:

Info

Title:
Establishment of Regeneration System of ‘Xinyu’ Grapevine in vitro
Author(s):
LUO Meng1WANG Jie1LI Yuling2SONG Shiren1ZHAO Liping1XU Wenping1
(1.School of Agriculture and Biology,Shanghai Jiao Tong University,Shanghai 200240;2.Grape and Melons Research Institute of Xinjiang Uygur Autonomous Region,Shanshan,Xinjiang 838200)
Keywords:
‘Xinyu’ grapevineregenerationexplanthormonelight quality
PACS:
-
DOI:
10.11937/bfyy.201609029
Abstract:
Taking ‘Xinyu’ grapevine as test material,using regeneration in vitro method,the effect of hormone combination IBA+6-BA and IBA+TDZ on callus induction and adventitious bud regeneration of leaves,petioles,and stem was studied.In addition,the inducing efficiency and differentiation potential of callus had been evaluated under six light conditions(red light,white light,blue light,red light after dark culture,white light after dark culture,blue light after dark culture) to find the most suitable light condition for the in vitro regeneration system of ‘Xinyu’ grapevine.The results showed that leaves should be used as explants for the regeneration of ‘Xinyu’ grapevine.The most suitable hormone combination was IBA 0.2 mg/mL+6-BA 2.0 mg/mL,while the most suitable light quality was red light(with 3-week dark culture).

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Last Update: 2016-07-07