Tissue Culture of Vaccinium corymbosum ‘O’Neal’(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Author(s):: LI Sen1; GAO Lixia2; LIU Nian3; ZHANG Shijun3; (1.Science and Technology Department，Zhongkai University of Agriculture and Engineering，Guangzhou，Guangdong 510225；2.Institute of Health and Agriculture，Zhongkai University of Agriculture and Engineering，Guangzhou,Guangdong 510225；3.College of Horticulture and Landscape Architecture，Zhongkai University of Agriculture and Engineering，Guangzhou,Guangdong 510225)

Abstract:: Taking stems with buds of Vaccinium corymbosum ‘O’Neal’ as explants，the effect of different combination of ZT，6-BA，NAA and IBA added into the media were tested.The results showed that the optimal disinfection treatment was soaking into 0.1% HgCl2 for 4 min with induction rate of 71.4%.The optimal medium for bud induction was WPM+1.0 mg/L ZT on which some branches germinated and plantlet length reached to be 4.7 cm.The suitable proliferation medium was WPM+2.0 mg/L ZT and the proliferation coefficient achieved to be 13.0.The optimum medium for root induction was 1/2WPM+0.1 mg/L IBA.The plantlets reached to be 5 cm in length and could be transplanted outside and the survival rate exceeded 80%.

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Last Update: 2016-07-06