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Optimization of Fermentation Conditions Mycelium Production by Cordyceps militaris Using Response Surface Methodology(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2016年08
Page:
138-141
Research Field:
Publishing date:

Info

Title:
Optimization of Fermentation Conditions Mycelium Production by Cordyceps militaris Using Response Surface Methodology
Author(s):
QIN Peng; WANG Long; LU Dengxue; HAN Rongbing; ZHAO Yuhui
(Institute of Biology，Gansu Academy of Sciences，Lanzhou，Gansu 730000)
Keywords:
Cordyceps militaris; mycelium; response surface; cordycepin
PACS:
-
DOI:
10.11937/bfyy.201608038
Abstract:
It was experimented to screen suitable temperature for Cordyceps militaris mycelium.After that，liquid medium was optimized by response surface method.Finally，using the optimized conditions，the mycelium was staticly fermented 10 days after 6 days shaking-flask culture，compared with 6 days shaking-flask culture,in order to understand production of Cordyceps militaries mycelium under different culture conditions and the effect of static fermentation on cordycepin production.The results showed that optimal conditions by one-factor experiment were dark at 25℃.Optimal liquid medium by response surface was sucrose 4.34%，yeast extract 3.06%，FeSO4 0.027%，KH2PO4 0.2%，(NH4)2SO4 0.04%，MgSO4 0.13%，vitamin B1 0.08%，ZnSO4 0.06%.The yield of mycelium by cordyceps militaris in the optimal medium increased 1.73 times compared with that in initial medium and reached 2.568 g/100mL,while the yield of mycelium reached 3.389 g/100mL with two-stage culture.And the results of two-stage culture showed that the yield of cordycepin increased 1.56 times more that in initial medium and reached 862.893 μg/mL，compared with 568.329 μg/mL of only 6 days shaking-flask culture.

References:

 

[1]LIN Y W，CHIANG B H.Anti-tumor activity of the fermentation broth of Cordyceps militaris cultured in the medium of Radix astragali[J].Process Biochemistry，2008，43(3)：244-250.

[2]REN Z H，CUI J H，HUO Z R，et al.Cordycepin suppresses TNF-α-induced NF-κB activation by reducing p65 transcriptional activity，inhibiting IκBα phosphorylation，and blocking IKKγ ubiquitination[J].International Immunopharmacology，2012，14(4)：68-703.

[3]CHEN Y，YANG S H，HUENG D Y，et al.Cordycepin induces apoptosis of C6 glioma cells through the adenosine 2A receptor-p53-caspase-7-PARP pathway[J].Chemico-Biological Interactions，2014，216(5)：17-25.

[4]林群英，宋斌，李泰辉.蛹虫草研究进展[J].微生物学通报，2006，33(4)：154-157.

[5]TULI H S，SHARMA A K，SANDHU S S，et al.Cordycepin：A bioactive metabolite with therapeutic potential[J].Life Sciences，2013，93(23)：863-869.

[6]钟思敏，杜梅，陈往滨，等.蛹虫草菌丝产虫草素液体培养条件的研究[J].菌物学报，2011，30(2)：229-234.

[7]周广麒，万晓星，侯友松，等.蛹虫草液态深层发酵的研究[J].食品与发酵工业，2004，30(8)：39-43.

[8]刘艳芳，唐庆九，顾俊杰，等.北冬虫夏草深层发酵高产虫草素工艺的优化[J].上海农业学报，2010，26(3)：26-30.

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Last Update: 2016-06-30