|Table of Contents|

The Establishment of Regeneration System of Tissue Culture of Artemisia annua L.(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2016年08
Page:
93-96
Research Field:
Publishing date:

Info

Title:
The Establishment of Regeneration System of Tissue Culture of Artemisia annua L.
Author(s):
LI Fei; MA Huanxin; LI Zhong’ai; WANG Zicheng
(College of Life Science,Henan University,Kaifeng，Henan 475004)
Keywords:
Artemisia annua L.; leaf; petiole; tissue cultivation; plant regeneration
PACS:
-
DOI:
10.11937/bfyy.201608026
Abstract:
In order to investigate the callus induction and the regeneration of the plant of Artemisia annua L.，to obtain rapid reproduction technology，the leaves and petioles of Artemisia annua L.were taken as explants,and the MS culture was chosen as the basic culture medium to be used in different combinations with hormone.The results showed that using leaves of Artemisia annua L.as explants，the callus induced medium was MS+2.0 mg/L 6-BA+0.5 mg/L 2,4-D，the induction rate was 78.0%；the differentiation medium was MS+1.0 mg/L 6-BA+0.05 mg/L NAA+3% sucrose+0.7% agar powder，the differentiation rate was 95.3%.The differentiated seedling rooted well in the culture medium 1/2MS+1.0 mg/L NAA,the rooting rate was 98.9%.Petioles of Artemisia annua L.could be a seedling by one step in medium MS+2.0 mg/L 6-BA+0.5 mg/L 2,4-D.The system could effectively shorten the training period，and was worthy of study.

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Last Update: 2016-06-24