Study on vitro Culture Technology by Axillary Bud Induction of Psammosilene tunicoides W.C.Wu et C.Y.Wu(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Title:: Study on vitro Culture Technology by Axillary Bud Induction of Psammosilene tunicoides W.C.Wu et C.Y.Wu

Author(s):: YANG Zexiong1; YIN Lisha2; ZHANG Jun3; TANG Junrong2; （1.Communism Youth League of Southwest Forestry University，Kunming，Yunnan 650224；2.Yunnan Forestry Senior Technical School，Kunming，Yunnan 650213；3.Key Laboratory for Forest Genetic and Tree Improvement and Propagation in Universities of Yunnan Province,Southwest Forestry University，Kunming，Yunnan 650224）

Abstract:: The tender stem with leaf axil of Psammosilene tunicoides was used as explants to select the basic culture medium，the best culture condition of axillary bud induction and multiplication culture.The transplanting and exercising of rooting seedling were also be carried out.The results showed that MS culture medium was suited to use as the basic culture medium to vitro culture of P.tunicoides.The good medium for axillary bud induction of P.tunicoides was MS+3.0 mg/L 6-BA+0.01 mg/L TDZ+0.20 mg/L NAA，the average induction buds was 4.15.In rooting meduinm of 1/2MS+0.30 mg/L IBA+0.10 mg/L NAA+0.3 g/L CA，the rooting rate was 89.6%；planting in the matrix of humus∶laterite∶perlite=1∶1∶1，the survival rate of P.tunicoides was 90.5%。

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Last Update: 2016-06-24