Genetic Diversity Analysis of Wild Ganoderma Strains Collected From Guangxi(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Title:: Genetic Diversity Analysis of Wild Ganoderma Strains Collected From Guangxi

Author(s):: CHEN Xuefeng; WEI Shiyan; WU Shengjin; WANG Canqin; WU Xiaojian; （Institute of Microbiology，Guangxi Academy of Agricultural Sciences，Nanning，Guangxi 530007）

Abstract:: Genetic diversity of ten wild Ganoderma strains collected from Guangxi was analyzed by using the technique of esterase isozyme analysis and antagonistic experiment.The results showed that there were rich genetic polymorphisms within the wild Ganoderma in Guangxi.22 bands were determined in the trail，and the bands among the strains were different.Cluster analysis demonstrated that the similarity coefficients ranged from 0.36 to 0.95 among the ten strains and were divided into 3 groups at the similarity coefficient of 0.55，which was Ganoderma lingzhi (strain C3)，Ganoderma lucidum（strain C9）and Ganoderma atrum（strains C1，C2，C4，C5，C6，C7，C8，C10）.The group of G.atrum could be divided into 2 subgroups (subgroup A included strains C1，C2，C4，C8 and C10，subgroup B included strains C5，C6 and C7） at the similarity coefficient of 0.64.Strains C4 and C10，as well as strains C5 and C7 could be considered as different isolates in the same strain，as they had high similarity coefficients above of 0.90 and without antagonistic activity between both strains.

[1]陈振妮,陈丽新,黄卓忠,等.广西灵芝产业现状及发展建议[J].食用菌,2015(1):4-6.

[2]贾定洪,郑林用,张小平,等.灵芝属38个菌株的酯酶同工酶研究[J].西南农业学报,2006，19(3):502-505.

[3]唐传红,张劲松,陈明杰,等.灵芝属菌株遗传多样性的初步研究[J].南京农业大学学报 2005，28(2)：133-136.

[4]潘丽晶,沈汉国,陈继敏，等.荧光AFLP和拮抗实验对灵芝菌遗传多样性研究[J].中国食用菌,2013,32(6):38-42.

[5]韦仕岩,王灿琴,覃小娟,等.金福菇菌株的酯酶同工酶分析[J].基因组学与应用生物学,2014,33(5):1025-1030.

[6]顾敏,沈颖越,金群力,等.双孢蘑菇SSR分子标记开发及其在遗传多样性分析中的应用[J].浙江农业学报,2013,25(5):987-993.

[7]王华,李渊,郭尚,等.5种食用菌遗传多样性的RAPD分子标记[J].山西农业科学,2015,43(5):516-517,547.

Last Update: 2016-06-16