Grinding Enzymolysis Purification and Microscopic Observation of Germination Characteristics on Chlamydospore of Agrocybe chaxingu(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Title:: Grinding Enzymolysis Purification and Microscopic Observation of Germination Characteristics on Chlamydospore of Agrocybe chaxingu

Author(s):: ZHANG Xiaomei; ZHU Weihong; MIAO Xiaoyan; WANG Mengmeng; (Department of Biochemistry，Baoding University，Baoding,Hebei 071000)

Keywords:: Agrocybe chaxingu; chlamydospore; enzymolysis conditions; germination position

Abstract:: With SM strain of Agrocybe chaxingu as the experimental material，by comparative studies of four separation methods and purification of Agrocybe chaxingu chlamydospore were conducted and its germination characteristics were observed.The results showed that the grinding enzymolysis method yielded better results under the condition of 1.0% snailase，1.0% cellulase，30℃ and 4 hours，the hyphal fragments enzymatically decomposed and disappeared，and the spores appeared plumpy with concentration up to 1.9×105/mL.Microscopic observations results found that the most chlamydospore germination happened on the flanks of the spore，and mycelium germinated with joint structures in lock shapes.The volumes of the spore vacuoles increased since germination while the spores were filled with large vacuoles.

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Last Update: 2016-06-15