|Table of Contents|

Study on Extraction Process of Total Saponins in Platycodon grandiflorum Stems With Aqueous Two-phase Cooperated With Microwave Method and Its Antioxidant Ability(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2016年07
Page:
119-124
Research Field:
Publishing date:

Info

Title:
Study on Extraction Process of Total Saponins in Platycodon grandiflorum Stems With Aqueous Two-phase Cooperated With Microwave Method and Its Antioxidant Ability
Author(s):
ZHONG Fangli1WANG Wenjiao12WANG Xiaolin1ZHAN Honghe1
(1.School of Chemistry and Pharmaceutical Engineering,Jilin Institute of Chemical Technology,Jilin,Jilin 132022;2.School of Chemistry,Jilin University,Changchun,Jilin 130012)
Keywords:
Platycodon grandiflorum stemsaqueous two-phasetotal saponinsantioxidant
PACS:
-
DOI:
10.11937/bfyy.201607029
Abstract:
Using Platycodon grandiflorum stems as experimental material,aqueous two-phase assisted with microwave method was applied to explore the influence of many factors on the total saponins extraction efficiency and its antioxidant ability in vitro.The results showed that the optimum ratio between material and solvent,the microwave power,the ratio between ethanol and water,extraction time and the mass concentration of ammonium sulfate were 1∶25 g/mL,600 W,0.77,7 min and 0.21 g/mL,respectively.Under optimum process condition,the average total saponins extraction efficiency reached 92.31%.The activity test showed that total saponins extraction solution from Platycodon grandiflorum stems had certain antioxidant ability in vitro,which might gradually enhance the clearance ratio with the increase of mass concentration of total saponins in extraction solution.

References:

 

[1]余椿声.桔梗[J].食品与药品,2006,82)75.

[2]梁仲远.桔梗水提液的镇咳、祛痰作用研究[J].中国药房,2011,22(35):3291-3292.

[3]吴葆华,吕望.桔梗皂苷D抑制人结肠癌SW620细胞增殖及其机制的研究[J].中国药学杂志,2013,48(5)354-358.

[4]杨柳娜,卢宗亮,周蕊,.桔梗皂苷D的杀精效果及促凋亡研究[J].中国计划生育杂志,2013,21(6):386-390.

[5]栾海艳,张建华,赵晓莲,.桔梗总皂苷对2型糖尿病肝病大鼠糖脂代谢影响的研究[J].中成药,2013,35(6):1307-1309.

[6]王茂山,吴敬涛.桔梗皂苷D对氧化型低密度脂蛋白诱导的内皮细胞氧化损伤的作用[J].食品科学,2013,34(13)29-296.

[7]于婷,李晓东,金乾坤,.桔梗提取物对小鼠的抗疲劳作用[J].食品工业科技,201233(24):394-396.

[8]欧丽兰,余昕,朱烨,.桔梗茎叶不同部位提取物的抗炎活性研究[J].安徽农业科学,2013,41(25):10272-10274.

[9]邓凡政,郭东方.芦丁在离子液体双水相中分配性能[J].应用化学,2007,24(7)838-840.

[10]高云涛,李干鹏,李正全,.超声集成丙醇-硫酸铵双水相体系从苦荞麦苗中提取总黄酮及其抗氧化活性研究[J].食品科学,2009,30(2):110-113.

[11]HATTI-KAUL R.Aqueous two-phase systems a general overview[J].Biomedical and Life Sciences,2001,19(3)269-277.

[12]LIU X HGAO Y TTANG R Set al. On the extraction and separation of iodide complex of cadmium() in propyl-alcohol ammonium sulfate aqueous biphasic system[J].Separation and Purification Technology200650(2)263-266.

[13]杨壮,田景奎,张琳.比色法测定桔梗中桔梗总皂苷的含量[J].中国现代应用药学,2008,25(1):58-60.

[14]李喜凤,薛秋萍,董诚明.桔梗中总皂苷的含量测定[J].中医药学刊,2006,24(12)2232-2234.

[15]张喜峰,杨春慧,罗光宏,.乙醇/硫酸铵双水相体系分离纯化葡萄籽总黄酮[J].食品与发酵工业,201339(10):254-258.

[16]张儒,张变玲,谢涛,.双水相体系萃取人参根中人参皂苷的研究[J].天然产物研究与开发,2012,24(11)1610-1613.

[17]邹家丽,邓骛远,和七一,.山茶花中多酚提取的方法及其抗氧化活性测定研究[J].北方园艺,2013(13):103-108.

[18]吕喜茹,郭亮,常明昌,.姬松茸粗多糖抗氧化作用[J].食用菌学报,2010,17(1):69-71.

Memo

Memo:
-
Last Update: 2016-06-15