Study on Tissue Culture and Rapid Propagation Technique of Parental Asparagus officinial L. of ‘Champion’(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Title:: Study on Tissue Culture and Rapid Propagation Technique of Parental Asparagus officinial L. of ‘Champion’

Author(s):: BAO Yancun; LI Shuhua; LI Baohua; ZHENG Hongxia; MU Meng; LI Guanghui; (Biotechnology Institute，Weifang Agricultural Science Institute,Weifang，Shandong 261041)

Keywords:: Asparagus officinial L.; ‘Champion’; shoot tip tissue; transplant

Abstract:: Taking parental Asparagus officinial of ‘Champion’ as materials，using different hormone combination of NAA,6-BA,KT,IBA,the start，proliferation，rooting，transition culture experiments were conducted and the suitable shoot-tip propagation medium for the parents were selected.The results showed that MS+NAA 0.05 mg/L+6-BA 0.5 mg/L+KT 0.1 mg/L as the start medium for the female parent；MS+NAA 0.10 mg/L+6-BA 0.5 mg/L+KT 0.2 mg/L as the start medium for the male parent；MS+NAA 0.1 mg/L+6-BA 1.5 mg/L+KT 0.1 mg/L as the subculture medium for the female parent；MS+NAA 0.2 mg/L+6-BA 1.5 mg/L+KT 0.05 mg/L as the subculture medium for the male parent；1/2MS+NAA 0.05 mg/L+6-BA 1.5 mg/L+KT 1.5 mg/L+IBA 1.5 mg/L as the rooting medium for the female parent；1/2 MS+NAA 0.1 mg/L+6-BA 1.5 mg/L+KT 1.0 mg/L+IBA 2.0 mg/L as the rooting medium for the male parent.In the appropriate rooting medium，the parents gave birth to eight or more roots of type I.Rooting rate and transplant survival rate were over 95%.

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Last Update: 2016-06-15