|Table of Contents|

Effect of Exogenous ABA on Activities and Isozymes of Antioxidant Enzymes in Anoectochius roxburghii(Wall.)Lindl.(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2016年07
Page:
93-98
Research Field:
Publishing date:

Info

Title:
Effect of Exogenous ABA on Activities and Isozymes of Antioxidant Enzymes in Anoectochius roxburghii(Wall.)Lindl.
Author(s):
ZHAO Xianlin; SI Qingyong; GONG Ning
(College of Life Sciences，Guizhou Normal University，Guiyang，Guizhou 550001）
Keywords:
Anoectochilus roxburghii(Wall.)Lindl.; ABA; antioxidant enzymes; isozyme
PACS:
-
DOI:
10.11937/bfyy.201607024
Abstract:
With the tissue cultured seedling of Anoectochilus roxburghii(Wall.)Lindl.as test material，using crude extract method,the effect of exogenous ABA on activities of antioxidant enzymes (SOD，POD，APX，and CAT) and isozymes was studied.The results showed that long time high concentration of ABA treatment on the activity of SOD and POD was significant；APX activity under low concentration of ABA treatment change was bigger，and APX enzyme activity reached the maximum under high concentration of ABA treatment for 24 hours；and CAT showed ‘M’ shifting trend under different concentration of ABA.By the analysis of isozymes，different concentration of ABA could induce new bands of POD and APX in specific time，but no new expression of SOD and CAT.

References:

 

[1]RAO M V，PALIYATH G,ORMROD D P,et al.Influence of salicylic acid on H2O2-metabolizing enzymes[J].Plant Physiol,1997,115:137-149.

[2]TAYLOR B，BURBIDGE A，THOMPSON A J.Control of abscisic acid synthesis[J].Exp Bot,2000,51:1563-1574.

[3]POPOVL P，TSONEV T D，LAZOVA G N，et al.Drought-and ABA-induced changes in photosynthesis of barley plants[J].Physiol Plant,1996,89:97-103.

[4]LEE T M，LUR H S，CHU C.Role of abscisic acid in chilling tolerance ofrice(Oryzasativa L.)seedlings.Ⅱ.Modulation of free polyamine levels[J].Plant Science,1997,16:1-10.

[5]WU Y，KUZMA J，MARECHAL E，et al.Abscisic acid signaling through cyclic ADP-ribose in plants[J].Science,1997,278:2126-2130.

[6]周伟香,龚宁,李凯,等.高温胁迫对金线兰生理特性影响的研究[J].贵州师范大学学报(自然科学版),2007,25(3):25-28.

[7]李亚军,周伟香,龚宁,等.低温胁迫对金线兰抗氧化酶活性的影响[J].贵州农业科学,2011,36(6):43-45.

[8]周黎,龚宁,彭健.干旱胁迫下金线兰渗透调节物质的变化规律[J].贵州科学,2008,26(4):68-71.

[9]王爱国,罗广华,邵从本,等.大豆种子超氧歧化酶的研究[J].植物生理学报,1983,9(1):77-84.

[10]张志良.植物生理学实验指导[M].北京:高等教育出版社,1990：88-91.

[11]DALTON D A,HANUS F J,RUSSELL S A,et al.Purification，properties,and distribution of ascorbate peroxidase in legumeroot nodules[J].Plant Physiol,1987,83：789-794.

[12]程鲁京,孟泽.钼酸铵显色法测定血清过氧化氢酶[J].临床检验杂志,1994,12(1)：6-9.

[13]BEAUCHAMP C,FRIDOVICH I.Superoxide dismutase：improved assays and an assay applicable to acrylamide gels[J].Analytical Biochemistry，1971,44:276-287.

[14]RAHNAMA1 H，EBRAHMZDEH H.Antioxidant isozymes activities in potato plants (Solanum tuberosum L.) under salt stress[J].Journal of Sciences，2006,17(3)：225-230.

[15]WOODBURY W,SPENCER A K,STAHMANN M A.An improved procedure using ferricyanide for detecting catalase isozymes[J].Analytical Biochemistry,1971,44：301-305.

[16]GILMOUR S J,ARTUS N N,THOMASHOW M F.cDNA sequence analysis and expression of two cold-regulated genes of Arabidopsis thaliana[J].Plant Molecular Biology,1992,18:13-21.

[17]李雪梅,张利红,何兴元,等.脱落酸对UV-C胁迫下小麦幼苗光合特性及抗氧化酶活性的影响[J].应用生态学报,2006，17(5):822-826.

[18]廖咏梅,母容,罗松青.外源脱落酸(ABA)对白术幼苗生长的影响[J].西华师范大学学报(自然科学版),2011,32(3):221-226.

[19]代其林,王劲,万怀龙,等.水杨酸对干旱胁迫下豇豆幼苗抗氧化酶活性的影响[J].四川大学学报(自然科学版),2008,45（5）:1258-1262.

[20]邢承华,李方,徐根娣,等.铁毒胁迫对水稻幼苗中POD和CAT同工酶的影响[J].浙江农业学报,2008,20(6)：457-460.

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Last Update: 2016-06-14