Different Factors on Callus Induction of Panax japonicus var.major(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Author(s):: LI Lixia; LI Tingting; ZHU Hong; SUN Changsheng; LIU Xuelan; ZHAO Houtao; (Guiyang Medicinal Botanical Garden，Guiyang,Guizhou 550002)

Abstract:: Taking knobs，internode and leaves，roots of winter buds of Panax japonicus var.major as experiment material，the effects of different disinfection method，explants，plant growth regulator and culture conditions on callus induction were studied to find the best method of callus induction.The results showed that the better disinfection treatment was 15 seconds with 75% C2H5OH，then 5 minutes with 0.2% HgCl2 for winter bud，but 15 minutes for knobs and internode.The internode was the best explant for callus induction.The induction rate 96.67% was the highest with 2,4-D 2.0 mg/L+KT 0.1 mg/L for internode.The MS medium with agar 5 g/L was suitable for induction of callus.Condition of the temperature 25℃，the photoperiod of 24 h/d and the light intensity of 6 200 lx was beneficial for callus induction.

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Last Update: 2016-06-14