|Table of Contents|

Cloning and Expression Analysis of BoPGIP2 Gene From Brassica oleracea(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2016年07
Page:
77-84
Research Field:
Publishing date:

Info

Title:
Cloning and Expression Analysis of BoPGIP2 Gene From Brassica oleracea
Author(s):
ZHAO Fengzhi1; WANG Xueying1; WANG Xinlei1; MA Xinwei1; JIANG Hanmin2; CHEN Chengbin1
(1.College of Life Sciences，Nankai University，Tianjin 300071;2.Tianjin Kernel Vegetable Research Institute,Tianjin 300384)
Keywords:
PGIP2 gene; Brassica oleracea; gene clone; expression analysis; Sclerotinia sclerotiorum
PACS:
-
DOI:
10.11937/bfyy.201607021
Abstract:
aking 3 strains Brassica napus as materials，using homologous cloning method，the PGIP2 gene of Brassica oleracea was cloned and the expression level was analysed.The results showed that BoPGIP2 had high similarity with PGIP2 gene of Brassica napus.The ExPASy analysis showed that molecular weight of BoPGIP2 was 37.02 kDa,theoretical pI was 8.51.The instability index (II) was 34.95，and this showed the protein was stable.Grand average of hydropathicity was -0.096，so it was not a hydrophilic protein.The subcellular localization analysis indicated that BoPGIP2 was a signal peptide of secretory pathway.qRT-PCR found that BoPGIP2 has high expression in stems and leaves in both high resistant varieties and resistant varieties，whereas BoPGIP2 expressed highly in root in susceptible varieties，it was consistent with their resistance to Sclerotinia sclerotiorum.Therefore，it might play an important role in the regulation of resistance to Sclerotinia sclerotiorum of Brassica oleracea.

References:

 

[1]李素文,赵前程,孙德岭,等.国内外花椰菜种植面积及产量变化趋势[J].中国蔬菜,2005(3):36-37.

[2]张凯,徐艳辉.花椰菜的分子遗传与育种研究进展概述[J].辽宁农业科学,2003(1):31-34.

[3]王燕,朱隆静,柳李旺,等.花椰菜生物技术育种研究进展[J].分子植物育种,2008(3):549-554.

[4]BOLAND G，HALL R.Index of plant hosts of Sclerotinia sclerotiorum[J].Can J Plant Pathol,1994（16）:93-108.

[5]PURDY L.Sclerotinia sclerotiorum：history diseases and symptomatology，host range，geographic distribution and impac[J].Phytopathology,1979,69:875-890.

[6]BOLTON M D T B.Sclerotinia sclerotiorum (Lib.) de Bary：biology and molecular traits of a cosmopolitan pathogen[J].Molecular Plant Pathology,2006(1):1-16.

[7]JAMAUX J，GELIE B，LAMARQUE C.Early stages of infection of rapeseed petals and leaves by Sclerotinia sclerotiorum revealed by scanning electron microscopy[J].Plant Pathol,1995,44:22-30.

[8]庞超,赵卫,谢永江.油菜菌核病的危害症状及防治对策[J].现代农业科技,2015(9)：137-142.

[9]杨子祥，苏银玲，沙毓沧.花椰菜菌核病的发生与防治技术[J].长江蔬菜,2012(21):46-47.

[10]王忠华,贾育林,夏英武.植物抗病分子机制研究进展[J].植物学通报,2004(5):521-530.

[11]HAMMOND-KOSACK K E，JONES J D J.Plant disease resistance genes[J].Annu Rev Plant Physiol Plant Mol Biol,1997，48(4):575-607.

[12]STASKAWICZ B J，AUSUBEL F M，BAKER B J，et al.Molecular genetics of plant disease resistance[J].Science，1995，268(5211):661-667.

[13]李文娴.PGIP的基因克隆及抗真菌活性研究[D].昆明:昆明理工大学,2010.

[14]HUANGFU H Y，GUAN C Y，JIN F R，et al.Prokaryotic expression and protein function of Brassica napus PGIP2 and its genetic transformation[J].Plant Biotechnology Reports，2014,8(2)：171-181.

[15]桂枝,高建明,袁庆华.多聚半乳糖醛酸酶抑制蛋白(PGIP)的研究进展概述[J].天津农学院学报,2011(3):36-41.

[16]罗娅,汤浩茹,张勇,等.草莓多聚半乳糖醛酸酶抑制蛋白基因(PGIP)的克隆及组织特异性表达[J].农业生物技术学报，2010(3)：424-430.

[17]陈夕军,张磊,陈羽,等.油菜Bnpgip2-1基因的克隆表达与生物信息学分析[J].中国油料作物学报,2014(6)：701-706.

[18]陈夕军,刘晓维,左示敏,等.水稻多聚半乳糖醛酸酶抑制蛋白基因(Ospgip1)原核表达及编码产物生物信息学分析[J].中国水稻科学，2011(2):136-142.

[19]符聪慧,王建平,张冲,等.苹果PGIP基因部分家族成员启动子的克隆与功能分析[J].园艺学报,2014,41(11):2169-2178.

[20]王秀菊.辣椒多聚半乳糖醛酸酶抑制蛋白基因克隆及功能研究[D].泰安:山东农业大学,2011.

[21]刘颖,吴剑锋,卢海宇,等.普通白菜PGIP基因BcPGIP分子特征研究[J].核农学报,2010(2):281-286.

[22]李广平,乔玉山,陶建敏,等.中国李PGIP基因的克隆及序列分析[J].西北植物学报,2006(9):1870-1873.

[23]李宽.花椰菜菌核病抗性评价体系的建立及抗性相关基因的表达研究[D].天津：南开大学,2014.

[24]HENRIK N，JACOB E，SREN B，et al.Identification of prokaryotic and eukaryotic signal peptides and prediction of their cleavage sites[J].Protein Engineering，1997（10）:1-6.

[25]OLOF E，HENRIK N，SREN B,et al.Predicting subcellular localization of proteins based on their N-terminal amino acid sequence[J].J Mol Biol,2000,300：1005-1016.

[26]MARCO B，STEFAN B，ANDREW W,et al.SWISS-MODEL：modelling protein tertiary and quaternary structure using evolutionary information[J].Nucleic Acids Research,2014,42:252-258.

[27]ARNOLD K，BORDOLI L，KOPP J，et al.The SWISS-MODEL workspace：a web-based environment for protein structure homology modelling[J].Bioinformatics,2006（22）：195-201.

[28]BENKERT P，BIASINI M，SCHWEDE T.Toward the estimation of the absolute quality of individual protein structure models[J].Bioinformatics，2011（27）:343-350.

[29]MILAGROS F M，FLORENCIA P O，GUSTAVO R D，et al.Isolation and characterization of a polygalacturonase-inhibiting protein from potato leaves：Accumulation in response tosalicylic acid，wounding and infection[J].Plant Physiology and Biochemistry,2001（2):129-136.

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Last Update: 2016-06-14