|Table of Contents|

Purification of Total Flavonoids From the Flowers of Salix caprea L.by the Macroporous Adsorption Resin(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2016年06
Page:
120-124
Research Field:
Publishing date:

Info

Title:
Purification of Total Flavonoids From the Flowers of Salix caprea L.by the Macroporous Adsorption Resin
Author(s):
FANG He; SONG Wangdi; CHEN Wen
(College of Pharmacy，Shihezi University，Shihezi,Xinjiang 832000)
Keywords:
Salix caprea flowers; macroporous resin; total flavonoids; purification technology
PACS:
-
DOI:
10.11937/bfyy.201606030
Abstract:
Taking Salix caprea L.as material，the optimum technological parameters of the purification process of total flavones from Salix caprea L.flowers with macroreticular resins was explored.The contents of total flavonoids were determined by using UV-Vis spectrum.Five kinds of resins were examined by static test.Purification conditions were investigated by using dynamic adsorption and desorption experiment.The results showed that AB-8 macroporus resin had good adsorption and desorption properties for favonoids in Salix caprea flowers.The optimal refined conditions were as follows：the velocity of sample solution 1.0 mL/min and pH 4.2，sample concentration of 3.13 g/L，the loaded amount of 5.5 mL/g，washing with distilled water 10 BV，then eluted with 6 BV of 70% ethanol at 2.0 mL/min.Under those optimum conditions，the purity of total flavonoids and the recovery was up to 51.99%.AB-8 macroreticular absorptive resin could effectively separate and purify total flavones from Salix caprea flowers，the purity coefficient thus was over 50%，which was in line with the study of effective components of herbal medicine.

References:

 

[1]何江，徐芳，陈燕，等.维吾尔药材黄皮柳花与其混淆品黄花柳花的鉴别研究[J].时珍国医国药，2010，21(8):1997-1998.

[2]居来提·托乎提，吐洪·艾买尔，吴秋灵.维吾尔医药爱维心口服液治疗冠心病心绞痛临床疗效观察试验研究[J].中国民族医药杂志，2009，4(4)：10-13.

[3]中国医学百科学编辑委员会.中国医学百科书(维吾尔医学)[M].上海:上海科学技术出版社，2005:210-211.

[4]ALAM M S，KAUR G，JABBAR Z，et al.Evaluation of antioxidant activity of Salix caprea flowers[J].Phytother Res，2006，20(6):479-483.

[5]TUNON H，OLAVSDOTTER C，BOHLIN L.Evaluation of anti- inflammatory activity of some swedish medicinal plants inhibition of prostaglandin biosynthesis and PAF-induced exocytosis[J].J Ethnopharmacol，1995，48(2)：61-76.

[6]SULTANA S，SALEEM M.Salix caprea inhibits skin carcinogenesis in murine skin：inhibition of oxidative stress，ornithine decarboxylase activity and DNA synthesis[J].Journal of Ethnopharmacology，2004，91(2)：267-276.

[7]AHMED A，SHAH WA，AKBAR S，et al.A short chemical review on Salix caprea commonly known as goa willow[J].Int J Res Phytochem Pharmacol，2011，1(1)：17-20.

[8]孙慧丽，艾来提·苏里坦，买吾兰·买提努尔，等.黄花柳中总黄酮含量的研究[J].新疆农业科学，2008，45(1):84-87.

[9]买吾兰江·买提努尔，艾来提·苏里坦，夏热帕提，等.响应面分析法优化超声波提取黄花柳总黄酮工艺[J].新疆农业科学，2009，46(4)：867-872.

[10]陈小川，冉继春，蒋瑶.大孔吸附树脂在中草药分离纯化中的应用[J].重庆中草药研究，2014(1):27-30.

[11]程振玉，宋海燕，杨英杰，等.AB-8大孔吸附树脂静态纯化北五味子木脂素工艺研究[J].北方园艺，2015(7)：141-145.

[12]闵建华，章振鹏，孟亚会，等.红景天苷大孔吸附树脂纯化工艺研究[J].中草药，2013，44(11)：1426-1430.

[13]柴建新，万茵，付桂明，等.杜仲叶总黄酮含量测定方法优化[J].中国食品学报，2013，13(4)：225-230.

[14]杨艳俊，王亚红，王君龙.景天三七总黄酮大孔吸附树脂纯化工艺研究[J].北方园艺，2014(7)：144-147.

[15]朱梦良，招丽君，梁新丽，等.大孔吸附树脂分离纯化葛根总黄酮和葛根素的工艺优选[J].中国实验方剂学杂志，2013，19(4)：23-26.

Memo

Memo:
-
Last Update: 2016-05-04