|Table of Contents|

Ethanol Reflux Method Extraction and Antioxidant Activities of Total Flavonoids From Lycopodii herba(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2016年06
Page:
116-120
Research Field:
Publishing date:

Info

Title:
Ethanol Reflux Method Extraction and Antioxidant Activities of Total Flavonoids From Lycopodii herba
Author(s):
YANG Shenming; FAN Shuguo; WANG Zhenji; YANG Zezhi
(Department of Chemistry and Life Science，Chuxiong Normal University，Chuxiong，Yunnan 675000)
Keywords:
Lycopodii herba; total flavonoids; extraction process; antioxidant properties
PACS:
-
DOI:
10.11937/bfyy.201606029
Abstract:
The optimal ethanol reflux extraction conditions of total flavonoids from Lycopodii herba were studied.In order to increase the extraction yield of total flavonoids，the ethanol reflux extraction conditions of total flavonoids from Lycopodii herba was optimized by orthogonal test L9 (34) based on the single-factor tests.Meanwhile，the antioxidant activity of total flavonoids from Lycopodii herba was evaluated by means of the scavenging capacity of the hydroxyl free radical and the DPPH free radical.The results showed that the optimum extraction parameters were 75% of the volume fraction of ethanol，60∶1 mL/g of liquid material ratio，150 minutes of extraction time，80℃ of extraction temperature，and under these conditions，the average extraction rate of total flavonoids was 1.82%.When the concentration of total flavonoids was 0.014 0 mg/mL，the scavenging rates of total flavonoids on ?OH and DPPH? were 72.65% and 93.02%，respectively，which implied that the total flavonoids had good ability to scavenge ?OH and DPPH?.

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Last Update: 2016-05-04