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Identification of Powdery Mildew of Potentilla supine(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2016年06
Page:
112-115
Research Field:
Publishing date:

Info

Title:
Identification of Powdery Mildew of Potentilla supine
Author(s):
PEI Dongli1; LIU Xiaofei2; CHEN Ke2
(1.Key Laboratory of Plant-microbe Interactions，Department of Life Science，Shangqiu Normal University，Shangqiu，Henan 476000；2.Shangqiu First Senior High School，Shangqiu，Henan 476000)
Keywords:
Potentilla supina; powdery mildew; ITS sequences; molecular systematics identification
PACS:
-
DOI:
10.11937/bfyy.201606028
Abstract:
Taking infected leaves of Potentilla supine as material，using etiological and molecular systematic identification methods，the phylogenetic evolution relationship of powdery mildew on Potentilla supine was studied in Shangqiu，Henan of China.The results showed that conidiophores of pathogenic fungi were cylindrical，vertical，simple and unbranched，measuring (90-180) μm×(10-17) μm；conidia revealed hyaline，ellipsoid，possessing conspicuous fibrosin bodies measuring (17-33) μm×(13-18) μm，and 4-6 conidia formed in chains；pathogenicity of inoculated leaves had no difference with leaves in natural state，symptoms began as white mycelium partially covering upper leaf surfaces；as the disease progressed，it spread to cover entire leaf surfaces；internal transcribed spacer (ITS) sequence of its ribosomal DNA was amplified by PCR and sequenced to obtain the 563 bp target fragment (GenBank accession No.KR049083).Sequence analysis by MEGA 3.1 software revealed that ITS sequence and one sequence from Herb eupatorii，Podosphaera fusca (GenBank accession No.JX548297) gathered together，distantly related to the ITS sequence of other four species in the same genus.The research showed that powdery mildew pathogen of Potentilla supina in Shangqiu was Podosphaera fusca.

References:

 

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Last Update: 2016-05-04