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Change of Several Enzymes Activity in Leaf of Cucumber After Inducted With FOC-RA-5(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2016年06
Page:
1-5
Research Field:
Publishing date:

Info

Title:
Change of Several Enzymes Activity in Leaf of Cucumber After Inducted With FOC-RA-5
Author(s):
LIU Yongsheng1; WANG Yong2; QIN Lijin3; JU Lingjun1; LIU Yan2; YUN Xingfu1
(1.College of Agronomy，Inner Mongolia Agricultural University，Huhhot，Inner Mongolia 010019；2.Vegetable Research Institute，Inner Mongolia Academy of Agriculture and Animal Husbandry，Huhhot，Inner Mongolia 010031；3.Chifeng University，Chifeng，Inner Mongolia 024000)
Keywords:
Fusarium wilt of cucumber; attenuated strain; induced resistance; disease index
PACS:
-
DOI:
10.11937/bfyy.201606001
Abstract:
t was conducted that germinated seed of cucumber at the time of radicle length of about 1 cm(soaking the radicle) and the cotyledons flattened(root irrigation) were induced two times with the pathogenicity of 0 FOC-RA-5，that treated with sterile water as the control.After that，the leaves were randomly selected as the test materials every 24 hours.These materials were used to measure cucumber phenylalanine ammonia lyase(PAL)，polyphenol oxidase(PPO)，peroxidase(POD)，lipid peroxidation (LOX)，ascorbate peroxidase (APX) and nitrate reductase (NR) activity in order to study the mechanism of resistance，which refered to the attenuated strain of cucumber against Fusarium wilt of cucumber.The results showed that the activity of enzymes LOX，PPO，PAL and POD in leaf increased at first and then decreased.The activity of enzymes NR and APX decreased at first and then increased.Enzyme activity in leaf of treated group was significantly higher than that of control group.The activity of enzymes APX，LOX，PAL,PPO,POD in leaf was negatively correlated with disease index of the induced plants.The activity of enzymes NR in leaf was positively correlated with disease index of the induced plants.

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Last Update: 2016-04-27