|Table of Contents|

Comparison of Agronomy Properties and DNA Barcoding Identification in Four Different Species of Lonicera(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2016年05
Page:
137-140
Research Field:
Publishing date:

Info

Title:
Comparison of Agronomy Properties and DNA Barcoding Identification in Four Different Species of Lonicera
Author(s):
LI Shaoji; JIANG Xianghui
(College of Chemistry and Materials Engineering，Kaili University，Kaili，Guizhou 556011)
Keywords:
Lonicera; Lonicera japonica; agronomic traits; DNA barcoding
PACS:
-
DOI:
10.11937/bfyy.201605036
Abstract:
Taking four species of Lonicera as test materials，the agronomical characters of four species of Lonicera，such as the height of plant，leaf length，leaf width，leaf shape and flowering period were studied.The results showed that character difference of four species was not obvious，which easily caused misjudgment in species identification.At the same time，the genomic DNA was analyzed by agarose gel electrophoresis and PCR amplification of DNA barcode was carried out for further comparison with five pairs of primers.The phylogenetic relationship of the four species by DNA barcoding sequencing was analyzed，and the phylogenetic dendrogram was built by MEGA.The results showed that there was no obvious difference between the agronomy traits of these four species，which easily caused misjudgment in species identification.On the basis of agronomical characters and DNA barcoding identification technology，the genetic diversity of the four species was analyzed，the result showed the dentification of DNA barcoding and the comparison of agronomical characters should be considered together when identifying the genetic relationship of species.

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Last Update: 2016-04-27