|Table of Contents|

Kinetics in Suspension Cell Culture of Achyranthes bidentata Bl.(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2016年05
Page:
132-136
Research Field:
Publishing date:

Info

Title:
Kinetics in Suspension Cell Culture of Achyranthes bidentata Bl.
Author(s):
LI Jinting1; GUO Xiaoshuang1; WANG Zhaoyang2; HAN Xueping1; ZHOU Zhiqiang1; ZHU Kaili1
(1.College of Life Sciences,Henan Normal University/Engineering Technology Research Center of Nursing and Utilization of Genuine Chinese Crude Drugs，Xinxiang，Henan 453007;2.Chinese Drugs Development Office in Nanzhao County，Nanyang，Henan 474650)
Keywords:
Achyranthes bidentata Bl.; suspension cell; cell growth; oleanolic acid
PACS:
-
DOI:
10.11937/bfyy.201605035
Abstract:
Taking the cell culture of Achyranthes bidentata Bl. as material，using HPLC,atomic absorption spectrometry method and conductivity measurement，the kinetic relationships of suspension cell growth，triterpenoid saponins production and nutrient consumption were analyzed.The results showed the suspension cell culture cycle lasted about 24 days by which the maximum biomass in dry weight and the oleanolic acid content reached to 13.47 mg/L and 1.418 mg/g.The kinetics analysis for suspension cell culture of A.bidentata Bl. showed that triterpenoid saponins production was increasing with the cell growth during a cell subculture cycle.The electrical conductivity of the culture medium gradually descended during culture procedure，reached to the lowest point on the 24th  day，and then slightly increased.The pH value declined and then increased.After 15 days of the cell culture，sucrose were consumed in culture medium.The large amounts of nitrogen，phosphate，K+ and Mg2+ were consumed on the 21st days.Compared with K+，Ca2+ could meet the needs of the cell growth.The results suggested that the nutrient deficiencies in medium would limite the cell rapid multiplication and triterpenoid saponins content accumulation.

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Last Update: 2016-04-27