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Study on Cryopreservation of Shoot Tips From in vitro Plants of Chrysanthemum by Vitrification Method(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2016年05
Page:
128-132
Research Field:
Publishing date:

Info

Title:
Study on Cryopreservation of Shoot Tips From in vitro Plants of Chrysanthemum by Vitrification Method
Author(s):
ZHANG Yanqiu; QU Lianwei; SU Junwei; XING Guimei; LU Jiaojiao; ZHANG Xiaofei
(Institute of Floriculture,Liaoning Academy of Agricultural Sciences,Shenyang，Liaoning 110161)
Keywords:
Chrysanthemum; tissue culture; shoot tips; cryopreservation
PACS:
-
DOI:
10.11937/bfyy.201605034
Abstract:
Chrysanthemum ‘Jinba’ was used as test material，and the experiment design of single factor optimization was used to research the effect of sucrose concentration in pre-culture medium，time of pre-culture，loading，exposure of PVS-2，size of shoot tips and the time of frozen on cryopreservation.The results showed that the optimal treatment combination realizing long-period preservation of the shoot tip of Chrysanthemum by vitrification method including：0.25 mol/L of sucrose in pre-culture medium，pre-culturing 3 days，loading treatment at 25℃ for 40 minutes，vitrification process at 0℃ for 60 minutes and 1.5-2.0 mm of shoot tips.The survival rate of shoot tips preservated for 180 days using this system could reach 71.33% after unloading and recovery training.

References:

 

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Last Update: 2016-04-27