|Table of Contents|

Organogenesis and Regeneration of Symplocos paniculata From Immature Zygotic Embryos in vitro(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2016年05
Page:
119-123
Research Field:
Publishing date:

Info

Title:
Organogenesis and Regeneration of Symplocos paniculata From Immature Zygotic Embryos in vitro
Author(s):
YANG Yan1; JIANG Lijuan2; TANG Yuxi1; LI Changzhu1; TANG Jie1; LI Yongjin1
（1.Hunan Forestry Academy，Changsha，Hunan 410004;2.College of Life Science and Technology,Central South University of Forestry and Technology，Changsha，Hunan 410004)
Keywords:
Symplocos paniculata; immature zygotic embryos; organogenesis; regeneration
PACS:
-
DOI:
10.11937/bfyy.201605032
Abstract:
Taking immature zygotic embryos of Symplocos paniculata as materials，using modified MS medium,the organogenesis and regeneration were researched.The results showed that the mMS medium could induce callus greatly，the highest induction rate 92.5% was obtained on the medium with 0.2 mg/L 6-BA+0.1 mg/L NAA.72.4% of calluses initiated green bud-like structures when they were cultured on mMS plus 0.25 mg/L 6-BA+0.15 mg/L NAA.The calluses with green bud-like structures were subcultured onto mMS medium without plant growth regulators for 15 days，76.6% of them developed into shoots.Shoots elongated to 1.5 cm long on 1/2 mMS medium plus 1.5% sucrose 20 days after culture.

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Last Update: 2016-04-27