|Table of Contents|

Molecular Cloning and Expression of PyANS in ‘Pingguoli’ During the Pigmentation(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2016年05
Page:
108-112
Research Field:
Publishing date:

Info

Title:
Molecular Cloning and Expression of PyANS in ‘Pingguoli’ During the Pigmentation
Author(s):
WANG Xue; QU Baihong; LU Yanxin; YANG Hongxia
(College of Agronomy,Yanbian University,Yanji,Jilin 133002)
Keywords:
‘Pingguoli’; PyANS; gene cloning; sequence analysis; expression patterns
PACS:
-
DOI:
10.11937/bfyy.201605030
Abstract:
With the ‘Pingguoli’ debagged and not sack as test material,the full-length cDNA sequences of PyANS was cloned by homologous cloning technology and real-time fluorescent quantitative PCR technique,then to analyze the relationship between the expression and the accumulation of anthocyanin during the pigmentation of ‘Pingguoli’.Sequence analysis showed that the cDNA of PyANS was 1 075 bp in length,encoded 358 amio acids with a calculated molecular weight(MV) of 40 kDa and isoelectric point(pI) of 5.38.Homology analysis revealed that the similarity between these cDNA fragments and the genes of other rosaceous plants was up to 99%.Real-time fluorescent quantitative PCR indicated the expression levels of PyANS was markedly enhanced in the sunlit side,increased rapidly on the first day after bagging,then reached a maximum value at 10th day and started to decline quickly,however,expressed stably in the same level finally.The color code and anthocyanins content produced results essentially in agreement with these figures.In addition that the PyANS had a certain role in promoting the accumulation of fruit anthocyanin.

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Last Update: 2016-04-27