|Table of Contents|

Establishment of High Efficient Regeneration System of Pyrus ussuriensis Leaves(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2016年04
Page:
97-101
Research Field:
Publishing date:

Info

Title:
Establishment of High Efficient Regeneration System of Pyrus ussuriensis Leaves
Author(s):
WANG Defen; ZHANG Mei; LI Dingli; WANG Ran; MA Chunhui; SONG Jiankun
(College of Horticulture，Qingdao Agricultural University，Qingdao,Shandong 266109)
Keywords:
Pyrus ussuriensis; leaves; tissue culture; regeneration
PACS:
-
DOI:
10.11937/bfyy.201604026
Abstract:
In this study，an efficient regeneration system of leaves of Pyrus ussuriensis was established by using the NN69 basic culture medium.The results showed that introduction effect of TDZ was better than that of 6-BA and KT，introduction effect of IBA and IAA was better than NAA and 2，4-D，in adventitious bud regeneration.The optimal culture medium of shoot regeneration whose efficiency was 100.0% and the number of shoots per plant was 8.47，was NN69+TDZ 3.0 mg/L+IBA 0.3 mg/L.The optimal of root induction was 1/2MS+IBA 0.5 mg/L，and the efficiency of it was 45.5% and root number was 2.24 per shoot.

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Last Update: 2016-04-21