|Table of Contents|

Plant Regeneration From Hedychium coronarium via Thin Layer Culture(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2016年03
Page:
96-99
Research Field:
Publishing date:

Info

Title:
Plant Regeneration From Hedychium coronarium via Thin Layer Culture
Author(s):
XIAO Wang; TU Hongyan; ZHANG Ailing; WU Songbin; CHEN Huiyan
(Biology and Food Engineering Institute，Guangdong University of Education，Guangzhou，Guangdong 510303)
Keywords:
Hedychium coronarium; thin layer culture; plant regeneration
PACS:
-
DOI:
10.11937/bfyy.201603026
Abstract:
Taking Hedychium coronarium plantlet as explants，the effect of age of plantlets，layer position，concentration of plant grow regulators on bud inducing were researched by thin layer culture.The results showed that the highest frequency of shoot regeneration (9.5 per plant) was achieved using layers，which were proximal to apical meristems，and the age of plantlet of 28 days；an average of 9.9 or 12.8 shoot buds could be obtained from thin cell layers derived from each plantlet on medium with 3 mg/L 6-BA or 0.1 mg/L TDZ，respectively；the shoots could be successfully regenerated to plantlets on MS medium with 1 g/L active charcoal.Well rooted plantlets were successfully acclimatized with a survival rate 95%.

References:

 

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Last Update: 2016-04-12