Influencing on Factors of Proliferation and Differentiation of Rhizome and Plantlet Growth in Cymbidium ‘XiaoFeng’(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Title:: Influencing on Factors of Proliferation and Differentiation of Rhizome and Plantlet Growth in Cymbidium ‘XiaoFeng’

Author(s):: YANG Jing; XIE Li; ZENG Ruizhen; SHANG Ruixin; GUO Herong; ZHANG Zhisheng; （College of Forestry and Landscape Architecture，South China Agricultural University，Guangzhou，Guangdong 510642）

Keywords:: Cymbidium; rhizome; proliferation; differentiation; seedling strength

Abstract:: Taking Cymbidium ‘XiaoFeng’ as test material,the factors influencing on proliferation and differentiation of rhizomes and seedling strengthening were studied on Cymbidium ‘XiaoFeng’.The results showed that MS basic medium，sucrose 30 g/L，6-BA 2.0 mg/L and NAA 0.5 mg/L，activated carbon 0.5 g/L were conducive to proliferation of the rhizomes，while 20% coconut juice and tomato juice 40 g/L inhibited proliferation of the rhizomes.1/2MS basic medium，sucrose 30 g/L，6-BA 2.0 mg/L and NAA 0.2 mg/L，20% coconut juice，1 000 lx light intensity favored differentiation of the rhizomes.Activated carbon had significant effect on differentiation of the rhizomes，medium without activated carbon promoted bud differentiation and inhibited root differentiation，on the contrary，medium with activated carbon inhibited bud differentiation and promoted seedling differentiation.Mashed potato 60 g/L significantly promoted plantlet growth，and seedlings with 3-4 cm height were suitable for transferring after cultured on 1/2MS+6-BA 0.1 mg/L+NAA 0.5 mg/L+sugar 20 g/L+patato 60 g/L+activated carbon 0.5 g/L for 40 days.

[1]张志胜，欧秀娟.墨兰的组织培养[J].园艺学报，1995，22（3）：303-304.

[2]程芬芳，陈新荣，陈莹莹，等.墨兰的组织培养研究进展[J].黑龙江农业科学，2015（3）：155-159.

[3]李阳，杨鹏伟，丁遥，等.墨兰根状茎在气升式反应器中增殖及绿芽分化研究[J].中国农学通报，2014，30（19）：137-141.

[4]GAO R，WU S Q，PIAO X C，et al．Micropropagation of Cymbidium sinense using continuous and reactor systems[J].Acta Physiol Plant，2014，36（1）：117-124.

[5]朱根发，叶秀斌，陈明莉，等.培养基不同成分对墨兰根状茎分化成苗的影响[J].中南林学院学报，2003，23（5）：42-44，58.

[6]王衍安，徐瑛.培养条件对墨兰组培芽增殖和生长的影响[J].山东林业科技，1999（2）：15-17.

[7]丁雪珍，韩磊，张文静.墨兰增殖培养基的筛选研究[J].北方园艺，2009（8）：208-209.

[8]陈丽，潘瑞炽，陈汝民.墨兰原球茎生长的研究[J].热带亚热带植物学报，1999，7（1）：59-64.

[9]施福军，莫昭展，韦江萍，等.墨兰的无菌播种及根状茎的增殖研究[J].安徽农业科学，2008，36（32）：13968-13969.

[10]郑艳艳，朴炫春，高日，等.墨兰根状茎增殖及生物反应器培养的可行性研究[J].安徽农业科学，2011，39（28）：17225-17227.

[11]钟士传，王侠礼.中国兰花-墨兰微体快繁技术研究[J].北方园艺，2004（1）：56-57.

[12]CHANG C，CHANG W C.Effect of thidiazuron on bud development of Cymbidium sinense Willd in vitro[J].Plant Growth Regulation，2000，30（2）：171-175.

[13]傅雪琳，张志胜，何平，等.墨兰根状茎绿芽分化的研究[J].华南农业大学学报，2000，21（3）：53-55.

[14]陈兰芬，王晶，田亦平，等.墨兰组织培养根状茎分化技术研究[J].河北林果研究，2011，26（1）：22-24.

[15]项艳，於凤安，彭镇华.墨兰离体快繁研究[J].林业科学研究，2003，16（4）：434-438.

Last Update: 2016-03-18