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Screening of High Frequency Regeneration Materials Suitable for Chloroplast Transformation From Three Cultivars of Brassica oleracea var.acephala(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2016年02
Page:
92-99
Research Field:
Publishing date:

Info

Title:
Screening of High Frequency Regeneration Materials Suitable for Chloroplast Transformation From Three Cultivars of Brassica oleracea var.acephala
Author(s):
PING Lu1; 2; GU Defeng1; WEI Zhengyi2; ZHONG Xiaofang2; LIN Chunjing2; XING Shaochen2; WANG Yunpeng2
(1.College of Horticulture，Jilin Agricultural University，Changchun，Jilin 130118；2.Agro-Biotechnology Research Institute，Jilin Academy of Agricultural Sciences,Changchun，Jilin 130033)
Keywords:
Brassica oleracea var.acephala; adventitious bud induction; chloroplast transformation; selection; high frequency regeneration
PACS:
-
DOI:
10.11937/bfyy.201602025
Abstract:
Taking three Brassica oleracea var.acephala cultivars with different color，‘Zhouyehongxin’，‘Bai’ou’ and ‘Hong’ou’ as materials，the frequency of adventitious bud induction were studied and so as to provide explants for subsequent chloroplast transformation.The MS medium was served as basic medium added with different concentrations of 6-BA，NAA and IBA，respectively，with the addition of bialaphos sodium and spectinomycin.The results showed that the impact of cultivar on induction of hormone from leaves of Brassica oleracea var.acephala was more visible than that of adventitious buds.The induction frequency of adventitious buds would be highest when the media were added with concentration of 1.0 mg/L 6-BA and 0.1 mg/L NAA for the cultivars of three Brassica oleracea var.acephala and so-called optimal medium for the three cultivars.According to the order from high to low，the ranking of frequencies of adventitious bud induction for three cultivars was ‘Hong’ou’，‘Bai’ou’ and ‘Zhouyehongxin’.Cultivar ‘Hong’ou’，therefore，was considered to be the suitable explant for the genetic transformation.The minimum inhibitory concentration of bialaphos-sodium and spectinomycin in induction medium for ‘Hong’ou’ cultivar was 4 mg/L and 50 mg/L separately.These results provided technical support for chloroplast transformation of Brassica oleracea var.acephala to implement genetic improvement.

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Last Update: 2016-03-18