|Table of Contents|

Research on in vitro Micrografting of Malus hupehensis var.Pingyiensis(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2016年02
Page:
88-91
Research Field:
Publishing date:

Info

Title:
Research on in vitro Micrografting of Malus hupehensis var.Pingyiensis
Author(s):
ZHAO Kai1; 2; ZHANG Zhihong2; LI Jinghui1; LI Daxing1; LYU Meng1
(1.Department of Agricultural Engineering，Songyuan Vocational Technical College，Songyuan，Jilin 138005；2.College of Horticulture，Shenyang Agricultural University，Shenyang，Liaoning 110866)
Keywords:
micrograft; apple; Malus hupehensis var.pingyiensis; sRNA transmission
PACS:
-
DOI:
10.11937/bfyy.201602024
Abstract:
Taking ‘Pingyitiancha’ (Malus hupehensis var.pingyiensis) as rootstock and ‘Hanfu’(Malus domestica cv.‘Hanfu’) as scion，the effect of different concentrations of 6-BA，ages of scion，leaves number of scion，kinds of scion/rootstock on the grafting survival rate was researched.The results showed that MS+IAA 0.3 mg/L+6-BA 1.0 mg/L，rootstocks and scions were cultured for 40 days，with 2 leaves on the scion and ‘Pingyitiancha’ was used as the rootstock，the survival rate of micrografting was 81.3%.Those were the foundation of transferring sRNA from rootstocks to scions.

References:

 

[1]MURASHIGE T，BITTERS W，RANGAN T，et al.Technique of shoot apex grafting and its utilization towards recovering virus-free citrus clones[J].Horticultural Science,1972（2）:19-25.

[2]NAVARRO L.Application of shoot-tip grafting in vitro to woody species[J].International Symposium on Vegetative Propagation of Woody Species，1987,227:43-56.

[3]JONARD R，LUKMAN D，SCHALL F，et al.Early testing of graft incompatibilities in apricot and lemon trees using in vitro techniques[J].Scientia Horticulturae,1990，43：117-128.

[4]SINGH B，SHARMA S，RANI G，et al.In vitro micrografting for production of Indian citrus ringspot virus (ICRSV)-free plants of kinnow mandarin (Citrus nobilis Lour× C.deliciosa Tenora)[J].Plant Biotechnology Reports，2008（2）:137-143.

[5]KADAM J，KARALE A，GARAD B，et al.Micrografting in wood apple，recent trends in horticultural biotechnology，national symposium on biotechnological interventions for improvement of horticultural crops[J].New India Publishing Agency，2007（2）:371-373.

[6]程玉琴,韩振海,许雪峰，等.试管微嫁接早期鉴定小金海棠与苹果品种的亲和性[J].农业生物技术学报,2003（11）：472-476.

[7]王颖,商月惠,王玉霞，等.平邑甜茶与扎矮山定子杂交后代的胚胎发育特征研究[J].园艺学报，2008,35：1093-1100.

[8]KANEHIRA A，YAMADA K，IWAYA T，et al.Apple phloem cells contain some mRNAs transported over long distances[J].Tree Genetics and Genomes，2010（6）：635-642.

[9]陈蕾.梨组织培养及器内嫁接技术的研究[D].延吉:延边大学，2008.

[10]HAROLDSEN V，PAULINO G，CHI-HAM C L，et al.Research and adoption of biotechnology strategies could improve California fruit and nut crops[J].Calif Agr,2012，66(2):62-69.

[11]SANO T，ISONO S，MATSUKI K，et al.Vegetative propagation and its possible role as a genetic bottleneck in the shaping of the apple fruit crinkle viroid populations in apple and hop plants[J].Virus Genes,2008,373:298-303.

[12]HAROLDSEN V，SZCZERBA M，AKTAS H.Mobility of transgenic nucleic acids and proteins within grafted rootstocks for agricultural improvement[J].Front Plant Sci,2012（3）:39.

[13]周瑞金,杜国强,师校欣.标记基因nptⅡ在转基因苹果嫁接砧穗间无相互传导[J].园艺学报，2006（33）：1329-1330.

[14]FLACHOWSKY H，TRANKER C，SZANKOWSKI I，et al.RNA-mediated gene silencing signals are not graft transmissible from the rootstock to the scion in greenhouse-grown apple plants Malus sp[J].International Journal of Molecular Sciences，2012（13）：9992-10009.

[15]BULLEY S，WILSON F，HEDDEN P，et al.Modification of gibberellin biosynthesis in the grafted apple scion allows control of tree height independent of the rootstock[J].Plant Biotechnology Journal,2005（3):215-223.

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Last Update: 2016-03-18