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Molecular Identification of Suspected Wild ‘Nanhua’ Volvariella volvacea Strain Based on Internal Transcribed Spacer Sequence(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2016年01
Page:
126-130
Research Field:
Publishing date:

Info

Title:
Molecular Identification of Suspected Wild ‘Nanhua’ Volvariella volvacea Strain Based on Internal Transcribed Spacer Sequence
Author(s):
LIU Zhu1; CAI Aiqun1; LIU Jinghua1; HE Zhidan2; MENG Xiaodong1
(1.Yindong College of Life Sciences，Shaoguan University，Shaoguan，Guangdong 512005；2.College of Life Sciences，South China Agricultural University，Guangzhou，Guangdong 510640)
Keywords:
Volvariella volvacea; wild strain; ITS sequence; BLAST; molecular identification
PACS:
-
DOI:
10.11937/bfyy.201601033
Abstract:
A suspected wild ‘Nanhua’ Volvariella volvacea was picked beside the mountains next to Nanhua temple in Shaoguan city of Guangdong Province.The pure culture was obtained by tissue isolation and was named strain VNH.The strain was identified by molecular identification with ITS sequence cloning and analyzing and combine with the traditional morphological.The results showed that,VHN strain was wild ‘Nanhua’ V.volvacea.It provided an important basis on the studies of biological characteristics and domestication cultivation for the wild strain.Meanwhile，it had a positive significance to enrich V.volvacea species resources and to promote the development of Shaoguan culture and tourism.

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Last Update: 2016-03-02