|Table of Contents|

Molecular Detection of Seed Zucchini Virus Diseases in Shihezi and Xinhu Region in Xinjiang(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2016年01
Page:
102-106
Research Field:
Publishing date:

Info

Title:
Molecular Detection of Seed Zucchini Virus Diseases in Shihezi and Xinhu Region in Xinjiang
Author(s):
REN Chenrong; HAO Xiaojun; DU Yejuan; XIANG Benchun
（College of Agriculture，Shihezi University/Key Laboratory at University of Xinjiang Uygur Autonomous Region for Oasis Agricultural Pest Management and Plant Protection Resource Utilization,Shihezi，Xinjiang 832003）
Keywords:
seed zucchini; molecular detection; virus disease; mixed infection
PACS:
-
DOI:
10.11937/bfyy.201601027
Abstract:
In order to understand the occurrence of seed zucchini virus diseases in Shihezi and Xinhu region in Xinjiang，seed zucchini virus diseases were investigated regularly in the field by RT-PCR and observation.The results showed that there were four symptom types of virus diseases which were mosaic，yellow，malformation and fern leaves，but the mosaic symptom was the most common in seed zucchini planting areas.The four viruses were detected by PCR which was cucumber mosaic virus (CMV)，zucchini yellow mosaic virus (ZYMV)，watermelon mosaic virus (WMV) and papaya ringspot virus (PRSV-W).The total detection rate of CMV，ZYMV，WMV and PRSV-W were 33.3%，84.8%，75.8% and 21.2%，respectively.In the field，all of the samples were infected with 2 or more viruses with the rate of 66.7%，and the mixed infection of ZYMV and other viruses (CMV，WMV，CMV+WMV+PRSV-W) were the crucial problem in seed zucchini.

References:

 

[1]吴会昌．我国西葫芦育种的现状、问题与对策[J]．北方园艺，2006(2)：48-49.

[2]林石明，廖富荣，陈青，等.葫芦科作物种传病毒及其检疫重要性[J].植物检疫，2012，26(1)：52-61.

[3]王威麟，张昊，于祥泉，等．侵染西瓜的5种病毒ZYMV、WMV、TMV、SqMV和CMV的多重RT-PCR检测体系的建立与检测应用[J]．植物病理学报，2010，40(1)：27-32.

[4]刘卫荣，向本春，韩盛．新疆小西葫芦黄化花叶病毒的分子鉴定与序列分析[J]．西北农业学报，2008，17(4)：223-228.

[5]SINGH Z，JONES R A C，JONES M G K.Identification of cucumber mosaic virus subgroup Ⅰ isolates from banana plants affected by infectious chlorosis disease using RT-PCR[J].Plant Disease，1995，79(7):713-716.

[6]张永江．一步法RT-PCR检测小西葫芦黄花叶病毒[J]．植物检疫，2005，19(3)：141-142.

[7]文朝慧．利用RT-PCR方法检测甜瓜种子中南瓜花叶病毒[J]．植物保护，2010，36(3)：130-133.

[8]ADKINS S，WEBB S E，BAKER C A，et al.Squash vein yellow virus detection using nested polymerase chain reaction demonstrates that the cucurbit weed Momordica charantia is a reservoir host [J].Plant Disease，2008，92(7):1119-1123.

[9]刘娟娟．石河子加工辣椒病毒病的调查与研究[D]．石河子:石河子大学，2014.

[10]FLETCHER J D，WALLACE A R，ROGERS B T.Potyviruses in New Zealand buttercup squash (Cucurbits maxima Duch.)-yield and quality effects of ZYMV and WMV 2 virus infections[J].New Zealand Journal of Crop and Horticultural Science，2000，28(1)：17-26.

[11]SIMMONS H E，HOLMES E C，GILDOW F E，et al.Experimental verification of seed transmission of zucchini yellow mosaic virus[J].Plant Disease，2011，95(6):751-754.

[12]黄传贤，覃秉益，奚仲兴，等．从新疆哈密瓜分离到的烟草坏死病毒及其理化性质的研究[J]．植物病理学报，1984，14(3)：153-158.

[13]郑光宇，董涛．在新疆发生的小西葫芦黄化花叶病毒的研究初报[J]．植物病理学报，1991，21(1)：72.

[14]刘卫荣.北疆葫芦科作物Potyvirus属病毒种类的分子鉴定与遗传分化研究[D].石河子：石河子大学，2008.

Memo

Memo:
-
Last Update: 2016-03-02