Establishment of the System of Rapid Propagation and Bud Induction of Fittonia(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Title:: Establishment of the System of Rapid Propagation and Bud Induction of Fittonia

Author(s):: ZHAO Huan; ZHANG Li; (College of Agronomy，Ningxia University，Yinchuan，Ningxia 750021)

Abstract:: With fittonia verschaffeltii ‘Small flame’,‘White angel’ two varieties of shoot tips and stem segments as explants，the effect of different disinfection time，different C source，and proportion of growth regulators on explants was researched.The results showed that the contamination rate was the lowest after explants sterilization 15 minutes，and the ‘Small flame’，‘White angel’ stem segments contamination rate were 16.7% and 13.3%，respectively.The best buds induction medium of the ‘White angel’ was MS+6-BA 1.0 mg/L+NAA 0.2 mg/L，the optimal subculture medium was MS+6-BA 1.0 mg/L+NAA 0.1 mg/L.The better variety buds induced culture of ‘Small flame’ was MS+6-BA 2.0 mg/L+NAA 0.1 mg/L，and the best performance could be seen when the subculture medium was MS+6-BA 1.0 mg/L+NAA 0.2 mg/L.Following in the sucrose medium was replaced with sugar better.Two varieties performed well when the rooting medium was 1/2MS+IBA 0.1 mg/L.

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Last Update: 2016-03-02