|Table of Contents|

Optimization of Chromosome Mounting Technique and Karyotype Analysis of Chlorophytum laxum R.Br(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2016年01
Page:
76-79
Research Field:
Publishing date:

Info

Title:
Optimization of Chromosome Mounting Technique and Karyotype Analysis of Chlorophytum laxum R.Br
Author(s):
LI Lei; SHEN Jifen; HE Mengling; YAN Hanjing
(College of Traditional Medicine，Guangdong Pharmaceutical University，Guangzhou，Guangdong 510006)
Keywords:
Chlorophytum laxum R.Br; chromosome; karyotype analysis
PACS:
-
DOI:
10.11937/bfyy.201601020
Abstract:
Taking the root-tips of Chlorophytum laxum R.Br as test materials，using a conventional chromosome mounting technique to prepare the slice specimens of chromosomes and its karyotype was analyzed.The effect of sampling time，permanent，dissociation and staining time on cytological observation were analyzed.The results showed that the optimum materials should be achieved at 08:00 AM，pretreated with 0.05% colchicine for 3 hours，fixing chromosomes in Kano’s fluid，then dissociated in 1.0 mol/L hydrochloric acid at a temperature of 60℃ for 15 minutes.Karyotype analysis of C.laxum showed that the chromosome number was 16 and the karyotype formula was 2n=2x=16=6m+8sm+2st.3 pairs were metacentric(m)，4 pairs were submetacentric(sm)，and 1 pair were subtelocentric (st).The karyotype of C.laxum was type 2A and the karyotype asymmetry coefficient was 64.39%.

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Last Update: 2016-03-02