|Table of Contents|

Cloning and Sequence Analysis of Polygalac-turonase (PG) Promoters in Tomato(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2016年01
Page:
72-75
Research Field:
Publishing date:

Info

Title:
Cloning and Sequence Analysis of Polygalac-turonase (PG) Promoters in Tomato
Author(s):
ZHOU Liying1; BU Lulu1; YANG Chunlei1; HOU Siming2; TIAN Tian3; YANG Zheng’an1
（1.Gardening College,Yunnan Agricultural University，Kunming，Yunnan 650201；2.Department of Lifescience and Technique,College of Kunming,Kunming,Yunnan 650213；3.Extension Center of Agricultural Technology of Qiaojia County，Zhaotong，Yunnan 654600）
Keywords:
Solanum lycopersicum; polygalac-turonase gene; promoter
PACS:
-
DOI:
10.11937/bfyy.201601019
Abstract:
Taking tomato variety of‘Zhongshu No.4’as material,1 500 bp PG gene promoter was amplified in tomato leaves using PCR，and bioinformatics analysis of PG promoter sequences was conducted.The results showed that by PLANTCARE database and PLANTCARE database showed that PG gene promoter had number of typical core elements such as TATA-Box，CAAT-Box，photoresponse element，thermal response element，ethylene response element，regulatory element related to tissue specific activation，regulatory element involved in endosperm expression，element involved in defense and stress responsiveness，circadian rhythm controlling element and so on，all of which laid the groundwork for such research on the future regulation of PG genes.

References:

 

[1]BARKHEM T,HALDOSN L A,GUSTAFSSON J ,et al.Ps2 gene expression in HepG2 cells:complex regulation through crosstalk between the estrogen receptor，an estrogen-responsive element,and the activator protein 1 response element[J].Molecular Pharmacology,2002，61(6):1273-1283.

[2]路静,赵华燕,何奕昆,等.高等植物启动子及其应用研究进展[J].自然科学进展,2004(8):17-23.

[3]糜赛男,曹明富.植物组织特异性启动子的研究进展[J].生物学教学,2010，35:7.

[4]王纪.香蕉果实特异表达启动子的分离鉴定[D].海口:海南大学,2010.

[5]李孟娇.柑橘果实成熟特异启动子的分离与功能初步分析[D].临安:浙江农林大学,2013.

[6]寇晓虹,朱本忠,罗云波,等.番茄果实中乙烯与多聚半乳糖醛酸酶的关系[J].植物生理与分子生物学学报,2004(6):675-680.

[7]薛炳烨,束怀瑞.多聚半乳糖醛酸酶(PG)与果实成熟软化研究进展[J].山东农业大学学报(自然科学版),2002,33(2):252-256.

[8]魏潇,刘威生,刘宁,等.果实软化相关PG基因的进化分析和基因组定位[J].园艺学报,2011(9):1791-1799.

[9]何勇,尹俊,李连国.甜瓜多聚半乳糖醛酸酶（PG）基因反义表达载体的构建[J].内蒙古农业大学学报(自然科学版),2004(2):81-85.

[10]薛炳烨,束怀瑞.多聚半乳糖醛酸酶（PG）与果实成熟软化研究进展[J].山东农业大学学报(自然科学版),2002,33(2):252-256.

[11]黄永红,梅眉,曾继吾,等.甜瓜多聚半乳糖醛酸酶反义基因植物表达载体的构建及其转化烟草的研究[J].果树学报,2007,24(4):492-495.

[12]尹涛,张上隆,刘敬梅,等.果实特异性启动子研究现状及其应用[J].农业生物技术学报,2009(2):355-360.

[13]刘玉瑛,张江丽.几个真核生物启动子计算机预测数据库资源概述[J].湖南农业科学,2007(4):70-71,74.

[14]刘玉瑛,张江丽.真核生物启动子预测相关数据库资源概述[J].安徽农业科学,2007(24):7418-7419.

[15]胡翔,周建林.真核生物基因启动子的计算机预测及其软件资源[J].激光生物学报,2004(6):465-467.

[16]BUTLER J E F,KADONAGA J T.Enhancer-promoter specificity mediated by DPE or TATA core promoter motifs[J].Genes Dev,2001,15:2515-2519.

[17]JUVEN GERSHON T,HSU J Y,KADONAGA J T.Caudal，a key developmental regulator，is a DPE-specific transcriptional factor[J].Genes Dev,2008,22:2823-2830.

[18]BUTLER J E F,KADONAGA J T.The RNA polymerase II core promoter:a key conponent in the regulation of gene[J].Genes Dev,2002,16:2583-2592.

Memo

Memo:
-
Last Update: 2016-03-02