|Table of Contents|

Optimization of Submerged Culture Conditions for Production of Fibrinolytic Enzyme by Cordyceps militaris(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2015年24
Page:
143-146
Research Field:
Publishing date:

Info

Title:
Optimization of Submerged Culture Conditions for Production of Fibrinolytic Enzyme by Cordyceps militaris
Author(s):
LIN Qunying1; 2; FENG Yi3; JIANG Yan3; LONG Liangkun3; DING Shaojun3; SUN Xiaoming1; 2
(1.Nanjing Institute for the Comprehensive Utilization of Wild Plants，Nanjing，Jiangsu 210042；2.Jiangsu Hongfeng Fruit and Vegetable Co.Ltd.，Suqian，Jiangsu 223700；3.College of Chemical Engineering，Nanjing Forestry University，Nanjing，Jiangsu 210037)
Keywords:
Cordyceps militaris; fibrinolytic enzyme; nutritional conditions; submerged culture
PACS:
-
DOI:
10.11937/bfyy.201524039
Abstract:
Liquid culture conditions of carbon and nitrogen sources for production of fibrinolytic enzyme by a Codyceps militaris strain were optimized using fibrin plate method.The best carbon and nitrogen sources were sceened out by single factor method.Based on the outcomes of the single factor method，an othogonal test was designed to optimize the most suitable formula.The results showed that lactose was the best carbon source，with enzyme activity at 118.69 U/mL，followed by the control without carbon source supplement，having activity at 109.12 U/mL.The best nitrogen source was sildworm pupa powder，with the activity of 118.69 U/mL and followed by yeast extract，giving enzyme activities of 29.10 U/mL.There was no certain relevance between enzyme activities and mycelial biomass.The optimal medium for fibrinolytic enzyme production consisted of lactose 10 g/L，sildworm pupa powder 10 g/L，yeast extract 5 g/L.Highest activity of 142.26 U/mL was obtained.

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Last Update: 2016-02-17