Study on the Technique of in vitro Rapid Propagation of Vaccinium ashei‘Climax’(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Title:: Study on the Technique of in vitro Rapid Propagation of Vaccinium ashei‘Climax’

Author(s):: LI Sen1; GAO Lixia2; LIU Nian3; ZHANG Shijun3; (1.Science and Technology Department, Zhongkai University of Agriculture and Engineering, Guangzhou,Guangdong 510225; 2.Institute of Health and Agriculture,Zhongkai University of Agriculture and Engineering,Guangzhou,Guangdong 510225; 3.College of Horticulture and Landscape Architecture,Zhongkai University of Agriculture and Engineering,Guangzhou,Guangdong 510225)

Abstract:: Taking the stems with axillary buds from Vaccinium ashei‘Climax’ as materials,the induction,propagation,rooting and transplanting of the plantlets were studied to set up technique of in vitro rapid propagation in ‘Climax’.The results showed that the suitable medium for adventitious bud induction was WPM+ZT 0.5 mg/L,the suitable medium for bud proliferation was WPM+ZT 1 mg/L,the optimal medium for bud rooting was WPM+ZT 1.0 mg/L+IBA 0.5 mg/L.During the rooting culture period,sand was used to fix plantlets instead of agar so that the medium which stuck to the root system could be washed easily and the roots could be protected from damage when transplanting.The survival rate reached 80% after 60 days’ transplanting.

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Last Update: 2016-02-17