|Table of Contents|

Influence of Inoculation Density and Sucrose Concentration on Induction Rate of Anther of Platycodon grandiflorum(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2015年24
Page:
98-101
Research Field:
Publishing date:

Info

Title:
Influence of Inoculation Density and Sucrose Concentration on Induction Rate of Anther of Platycodon grandiflorum
Author(s):
YANG Li; HUANG Hua; JIN Jiangshan; YAN Yizi
(Agricultural College，Yanbian University,Yanji,Jilin 133002)
Keywords:
Platycodon grandiflorum; anther; concentrations of sucrose; inoculated density
PACS:
-
DOI:
10.11937/bfyy.201524028
Abstract:
Using the anther of Platycodon grandiflorum as explants，different density of anther were inoculated and different concentrations of sucrose were added respectively in the culture medium,and their effect on induction rate of anther was studied.The results showed that MS+1.0 mg/L 6-BA+0.5 mg/L NAA as induction medium，with the increase of density callus induction rate was decreased；MS+1.0 mg/L 6-BA+0.2 mg/L BA+2，4-D as induction medium，callus induction rate first increase and then decrease with the increase of density，when the density reach to 15，the induction rate was the highest；and the induction rate of the latter were significantly higher than that of the former induction rate.MS+1.0 mg/L 6-BA+0.5 mg/L NAA as induction medium，attaching different concentrations of sucrose，with the increase of sucrose concentration，callus induction rate showed a trend of rise after the fall of the first，while the concentration of sucrose reached to 60 g/L，the induction rate was the highest.

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Last Update: 2016-02-17