|Table of Contents|

Screening of DNA Barcode for Actinidia(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2015年24
Page:
79-83
Research Field:
Publishing date:

Info

Title:
Screening of DNA Barcode for Actinidia
Author(s):
YE Chanjuan1; YANG Miaoxian1; LIU Wen1; 2; LIANG Hong1
（1.College of Life Sciences，Zhongkai University of Agriculture and Engineering，Guangzhou，Guangdong 510225；2.College of Agriculture，South China Agricultural University，Guangzhou，Guangdong 510642）
Keywords:
Actinidia; wild species; DNA barcoding; screening
PACS:
-
DOI:
10.11937/bfyy.201524023
Abstract:
DNA barcode technology could used for the kiwifruit breeding usually.In this study，six DNA barcode，rbcL，matK，psbA,trnL-F，ITS and trnH-psbA were used to identify five wild species of Actinidia.The results showed that，GC contents of trnL-F and ITS were higher，with the range from 51.1% to 55.2%，while trnH-psbA had the lowest GC content(32.0%).Above all six DNA barcode markers were blasted in NCBI (National Center for Biotechnology Information)，the results showed that the sequences similarity was more than 99% between the wild species of Actinidia and the species of Actinidia logged on NCBI，and there were different similarity levels among the different DNA barcode.Thus，these results revealed the intraspecific diversity of Actinidia or penetration among different species of Actinidia.Then，the ITS marker could be used to identify all the above 5 species of Actinidia according to the NJ (Neighbor-Joining) tree.And the evolutionary divergence among 5 species was obvious in ITS sequences.The trnL-F，ITS and matK barcode had higher diversity of nucleotide and Tajima’s test value.By the means of sequence analysis with the six DNA markers，ITS presented excellently in variation of Actinidia，because of its higher nucleotide diversity and obvious difference among different taxa.

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Last Update: 2016-02-17