|Table of Contents|

Cloning and Sequence Analysis of RNase-like Gene From Citrus grandis var.Shatinyu Hort(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2015年24
Page:
70-74
Research Field:
Publishing date:

Info

Title:
Cloning and Sequence Analysis of RNase-like Gene From Citrus grandis var.Shatinyu Hort
Author(s):
LIU Yujie; GUO Danni; ZHANG Yu; QIN Xinmei; LI Huimin; QIN Xinmin
(College of Life Science，Guangxi Normal University/Key Laboratory of Ecology of Rare and Endangered Species and Environmental Protection，Guilin，Guangxi 541004)
Keywords:
Citrus grandis var.Shatinyu Hort; RNase-like gene; sequence analysis
PACS:
-
DOI:
10.11937/bfyy.201524021
Abstract:
The self-pollinated and cross-pollinated style of Citrus grandis var.Shatinyu Hort were used as test materials and transcriptome sequenced by high-throughput sequencing technology.The sequence of RNase-like storage protein was obtained by differential analysis，what’s more，the physical and chemical properties of this protein were studied.The result showed that,the length of this gene(GenBank accession number：KP172529) was 1 048 bp with an open reading frame(ORF)of 693 bp，encoding 290 amino acids with deduced molecular weight of 25.82 kDa，and the theoretical pI value was 5.30.And it belonged to the RNase-T2 superfamily with a conserved region binding the nucleotide.This protein was a hydrophobic protein，and there were 15 phosphorylation sites within the polypeptide chain.The homology analysis of amino acid sequence indicated that the RNase-like storage protein shared high homology with Citrus sinensis(99%) and Citrus clementina(99%).The phylogenetic tree revealed that RNase-like gene storage protein showed closer kinship with Citrus sinensis and Citrus clementina，indicating that they belonged to the same evolutionary branch.

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Last Update: 2016-02-16