Study on the Inhibitory Action of Apple Ring Spot in the Storage Period(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Title:: Study on the Inhibitory Action of Apple Ring Spot in the Storage Period

Author(s):: HOU Xiaojie1; LIANG Kuijing1; CONG Rizheng2; LU Zhangen1; （1.Department of Life Science，Hengshui University，Hengshui，Hebei 053000；2.College of Forestry，Agricultural University of Hebei，Baoding，Hebei 071000）

Keywords:: apple ring spot; culturable endophytic bacteria; rhizosphere soil; rivalry

Abstract:: Apple,garlic and ginger were used as test materials,the confrontation culture method was aolopted to study the antagonism effect from culturable endophytic bacteria within healthy apple fruit，ginger，garlic，and the rhizosphere soil microorganisms in healthy apple plant for the apple ring spot.The results showed that,nine kinds of bacteria in rhizosphere soil can be cultured；the number of strains isolated from ginger，garlic and apple was 5，3 and 1，respectively.All of the isolated microorganisms except ‘soil-2’ obtained from soil had the inhibition ability and isolates from the rhizosphere soil had greater proportion of antagonism，44.4%；followed by ginger and garlic，the proportion was 27.8% and 16.7%.All strains except ‘garlic 1 ②’，‘soil-1’ and ‘soil-5’ had significant difference comparing with CK.The ‘ginger 4’ had the best inhibitory effect，the diameter of fungal colonies was only (30.78±0.49) mm，‘soil-4’ was following，fungal colony diameter was (35.12±0.13) mm.

[1]百度百科.苹果轮纹病[EB/OL].http://wapbaike.baidu.com/view/230696.html.

[2]LI G X,SHEN Y B.Study on the infection mechanism of apple ring rot disease [J].Journal of Fruit Trees，2007,24(1):16-20.

[3]荔克让,张华,李晓云.苹果果实轮纹病及其防治[J].中国沙漠,2000,7(20):184-186.

[4]王光华.生防细菌产生的拮抗物质及其在生物防治中的作用[J].应用生态学报,2004,15(6):1100-1104.

[5]百度百科.根际微生物[EB/OL].http://wapbaike.baidu.com/view/446359.html.

[6]东秀珠,蔡妙英.常见细菌系统鉴定手册[M].北京:科学出版社,2001:25-27.

[7]宫春波,杨伟,刘永红,等.鲜姜汁抑菌效果的测定[J].中国调味品,2005(9):50-53.

[8]陈洪生.大蒜素提取条件的优化及其抑菌活力的研究[J].食品工业科技,2007,28(4):87-89.

〖LL〗[9]陈祥贵,裴炎,彭红卫.蜡状芽孢杆菌S-1菌株抗真菌物质的分离纯化[J].四川工业学院学报,2002(2):60-63.

[10]范青,田世平,蒋爱丽,等.采摘后果实病害生物防治拮抗菌的筛选和分离[J].中国环境科学,2001，21(4):313-316.

[11]鄂华.非寄主植物内生菌防治桉树青枯病研究[D].保定：河北农业大学,2009.

[12]汪茜,胡春锦.真菌病害拮抗菌的筛选及其对多种植物病原真菌的拮抗活性测定[J].广西农业科学2010,41(7):675-678.

[13]黄海东,李晓燕,杨红鹏,等.龙胆内生菌的分离鉴定及其对苹果腐烂病病菌的拮抗作用[J].食品工业科技,2012(6):217-218.

[14]陈亮,刘君丽.丁香菌酯对苹果树腐烂病的防治[J].农药,2009,4 (6):402-404.

[15]邓振山,赵龙飞,王薇薇,等.银杏内生真菌的分离及其对苹果腐烂病病原菌的拮抗作用[J].西北植物学报,2009,29(3):608-613.

[16]乔红萍,宏丽丽,康振生.小麦内生细菌及其对根部主要病原真菌的抑制作用[J].应用生态学报,2006,17（3):690-694.

[17]史光瑚.我国水果业的发展和几点建议[J].果树科学,1998,15(2):97-102.

[18]侯保林,王江柱,朱杰华,等.苹果果实轮纹病的发生和防治[J].中国果树,1998(2):42-43.

Last Update: 2016-01-15