|Table of Contents|

Establishment for SSR Reaction System of Pyrus ussuriensis Maxim and Its Stability Assessment(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2015年23
Page:
104-107
Research Field:
Publishing date:

Info

Title:
Establishment for SSR Reaction System of Pyrus ussuriensis Maxim and Its Stability Assessment
Author(s):
LI Honglian; WANG Qiang; YAN Xingkai; ZHANG Maojun
(Pomology Research Institute，Jilin Academy of Agricultural Sciences/Scientific Observing and Experimental Station of Pomology (Jilin，Northeast Region)，Ministry of Agriculture，Gongzhuling，Jilin 136100)
Keywords:
Pyrus ussuriensis Maxim; SSR marker; reaction system; primer screening
PACS:
-
DOI:
10.11937/bfyy.201523029
Abstract:
Genomic DNA of young leaves of Pyrus ussuriensis Maxim was tested as the template,optimization experiments on some important parameters affecting SSR-PCR amplification were conducted to establish an optimum system suitable for SSR-PCR Pyrus ussuriensis Maxim.Orthogonal design was used to optimize SSR anplification system with five factors （including DNA template，Mg2+，Taq polymerase，dNTPs and primer） at five levels，respectively.The results showed that，a suitable SSR-PCR reaction system was established，its total volume was 20 μL，containing 10 ng DNA template，2.5 μL Mg2+（20 mmol/L)，1.25 U Taq polymerase，1.0 μL dNTPs （10 mmol/L)，1.5 μL primer （10 μmol/L) and 2 μL 10×PCR buffer.The reaction system was successfully applied in primer screening experiment and the amplification of the lines from Pyrus ussuriensis MaximבKosui’ using primer CHg04b，and indicated the suitability and stability of the system.

References:

 

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Last Update: 2016-01-15