|Table of Contents|

Isolation Identification,Mycelial Cultivation Characteristics and Artificial Cultivation of a Wild Ganoderma gibbosum Strain(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2015年22
Page:
153-157
Research Field:
Publishing date:

Info

Title:
Isolation Identification,Mycelial Cultivation Characteristics and Artificial Cultivation of a Wild Ganoderma gibbosum Strain
Author(s):
ZHANG GuoguangLU WenpengLIN LiuzhangZOU Jinmei
(School of Biological Science and Technology,Minnan Normal University,Zhangzhou,Fujian 363000)
Keywords:
Ganoderma gibbosumcultivation characteristicsartificial domesticated cultivation
PACS:
-
DOI:
10.11937/bfyy.201522040
Abstract:
A wild macrofungi was taken as research object.The wild macrofungi was identified by internal transcribed spacer(ITS) sequence and homologous sequence alignments,simultaneously mycelial cultivation characteristics and artificial cultivation were investigated by solid plate culture method and artificial logs.The results showed that the isolating wild strain was Ganoderma gibbosum by phylogenetic analysis based on ITS region sequence.Optimum carbon was the glucose or fructose,and the optimum nitrogen source was extracts of yeast,and the optimum carbon nitrogen ratio was about from 10∶1 to 20∶1.The optimum growth temperature and optimum pH value were around 28℃ and 5.5 respectively.The fruit body development was observed from three kinds of cultivating substrate whose main materials were sawdust,corncob and cottonseed hull separately.

References:

 

[1]戴玉成.中国多孔菌名录[J].菌物学报,2009,28(3):315-327.

[2]赵继鼎,徐连旺,张小青.中国灵芝科的分类研究Ⅱ[J].真菌学报,1983,2(3):159-167.

[3]钟金霞,郭建荣,肖敏,.海南岛药用灵芝资源的调查研究[J].中国药学杂,1998,33(11):652-655.

[4]吴兴亮,戴玉成.中国灵芝图鉴[M].北京:科学出版社,20051-229.

[5]吴兴亮,宋斌,赵友兴,.中国药用灵芝及名称使用商榷[J].贵州科学,2013,31(1):1-17.

[6]钟金霞,何青,何顺志,.中国主要药用灵芝的鉴别[J].中国中药杂志,1998,23(2):75-76.

[7]KIM Y S,EO S K,OH K W,et al.Antimicrobial activity of water soluble components of Elfvingia applanata alone and in combination with quinolones[J].Mycobiology,2001,29(1):11-14.

[8]周忠波,马红霞,图力古尔.树舌(Ganoderma lipsiense)化学成分及药理学研究进展[J].菌物研究,2005,3(1):35-42.

[9]陈体强,徐洁,吴锦忠.福建省常见灵芝的氨基酸分析比较[J].海峡药学,2004,16(5):1-4.

[10]SAMBROOK J,FRITSCH E F,MANJATID T.Molecular cloning[M].A laboratory Manual.2nd ed,New York:Cold Spring Harbor Laboratory Press,1989.

[11]张国广,邹金美,郑琳,.一株野生大型真菌的ITS分析及生物学特征研究[J].云南民族大学学报,2010,19(6):395-399.

[12]邓春英.中国小皮伞属广义球盖组分类学研究[D].广州:华南理工大学,2012.

[13]GOTTLIEB A M,FERRER E,WRIGHT J E.rDNA analyses as an aid to the taxonomy of species of Ganoderma[J].Mycology Research,2000,104(9):1033-1045.

[14]苏春丽,唐传红,张劲松,.基于rDNA ITS序列探讨中国栽培灵芝菌株的亲缘关系[J].微生物学报,2007,47(1):11-16.

[15]兰玉菲,王庆武,安秀榕,.野生硫磺菌分离株ITS序列分析[J].食用菌学报,2012,19(2):31-34.

[16]匿名.真菌、地衣汉语学命名法规[J].真菌学报,1987,6(1):61-64.

[17]陈向东,曾念开,兰进.药用真菌有柄树舌菌丝培养特性的研究[J].中国中药杂志,2010,35(15):1939-1942.

[18]梁志群,陈子武.有柄灵芝菌丝生物学特性研究[J].中国农学通报,2011,27(18):164-167.

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Last Update: 2016-01-08