|Table of Contents|

Identification and Analysis of DXS Gene Fragment in Nervilia fordii(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2015年22
Page:
114-117
Research Field:
Publishing date:

Info

Title:
Identification and Analysis of DXS Gene Fragment in Nervilia fordii
Author(s):
HUANG Qionglin1; HE Rui2; ZHAN Ruoting2
(1.Guangdong Medical University，Zhanjiang，Guangdong 524023;2.Research Center of Chinese Medicinal Resource Science and Engineering，Guangzhou University of Chinese Medicine/Key Laboratory of Chinese Medicinal Resource From Lingnan，Ministry of Education，Guangzhou，Guangdong 510006)
Keywords:
Nervilia fordii (Hance) Schltr.; 1-deoxy-D-xylulose-5-phosphate synthase; bioinformatics; expression
PACS:
-
DOI:
10.11937/bfyy.201522030
Abstract:
1-deoxy-D-xylulose-5-phosphate synthase is the first rate-limiting key enzyme of plant MEP terpenoids biosynthesis pathway.Enhancing the expression of DXS gene can promote the biosynthesis of terpenoids in plants.Here，one DXS gene fragment was identified from the transcriptome data of Nervilia fordii (Hance) Schltr.and named as NfDXS using bioinformatics.NfDXS encoded 718 amino acids and the coding protein was hydrophilic protein with a molecular weight at 77.45 kDa.NfDXS protein contained a 1-deoxy-D-xylulose-5-phosphate synthase functional domain and none signal peptide and transmembrane region.Secondary structure prediction revealed NfDXS was a hybrid protein.The expression level of NfDXS gene in leaf was higher than in corm.The study would provide a firm foundation for regulating the synthesis of effective terpenoids by gene manipulation of DXS in N.fordii.

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Last Update: 2016-01-08