|Table of Contents|

Comparative of Methods for RNA Extraction From Plum (Prunus salicina Lindl.) Fruit Flesh(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2015年22
Page:
106-110
Research Field:
Publishing date:

Info

Title:
Comparative of Methods for RNA Extraction From Plum (Prunus salicina Lindl.) Fruit Flesh
Author(s):
XUAN Jiping; JIA Zhanhui; QIAN Meihua; ZHANG Jiyu; WANG Gang; GUO Zhongren
（Zhongshan Botanical Garden,Institute of Botany，Chinese Academy of Sciences，Nanjing，Jiangsu 210014）
Keywords:
plum (Prunus salicina Lindl.); fruit flesh; comparative; RNA; modified CTAB
PACS:
-
DOI:
10.11937/bfyy.201522028
Abstract:
To select the suitable method for total RNA extraction from oriental plum(Prunus salicina Lindl.) fruit，the performances of three extraction methods，trizol，plant total RNA extraction kit and modified CTAB were compared.Trizol and RNA plant extraction kit were determined to hardly extract integrate RNA.Modified CTAB was capable of efficiently removing polysaccharides.The lightness of 28S rRNA of the RNA isolated by modified CTAB was 1.5-2.0 times as much as that of 18S rRNA，with the OD260/OD280 value ranging from 1.8 to 2.1.The RNA productivities from 5 samples of fruit tissue were 770.96-1 029.04 ng/μL，respectively.The specific RNA bands obtained by RT-PCR indicate that the RNA extracted by modified CTAB was of high-quality and good-integrity at a higher productivity，thus completely suitable for further molecular biological research.

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Last Update: 2016-01-08