(1.College of Agriculture，Hebei University of Engineering，Handan,Hebei 056021；2.Beijing Vegetable Research Center，Beijing Academy of Agriculture and Forestry Sciences/Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (North China)，Ministry of Agriculture/Key Laboratory of Urban Agriculture (North)，Ministry of Agriculture，Beijing 100097)
The study described an efficient in vitro micropropagation protocol for induction of multiple shoots from shoot tip explants of Cucurbita pepo L.The results showed that shoot tip explants were treated with 10% solution of sodium hypochlorite，which contain 0.02% mercury chloride (HgCl2)，for 10 minutes，the lowest contamination rate was 6.7%，the highest survive rate was 91.7%.The highest frequency (100%) of multiple shoot formation with maximum number of shoots (2.38 shoots per explants) was achieved on MS medium supplemented with 0.5 mg/L 6-BA and 0.10 mg/L IBA.Shoots were rooted most effectively in 0.30 mg/L indole-3 butyric acid (IBA)-supplenmented MS medium，and all rooted shoots were successfully transplanted into soil.