|Table of Contents|

Optimization Technology of Extracting Anthocyanins From the Purple Leaf Brassica napus by Orthogonal Experiment(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2015年21
Page:
125-128
Research Field:
Publishing date:

Info

Title:
Optimization Technology of Extracting Anthocyanins From the Purple Leaf Brassica napus by Orthogonal Experiment
Author(s):
HUANG Huiying1; WU Guoxing2; ZHENG Lijun1; LI Haibo1
(1.College of Yingdong Agricultural Science and Engineering，Shaoguan University，Shaoguan,Guangdong 512005；2.Guangdong Jinyou Group Co.Ltd.，Shaoguan,Guangdong 512005)
Keywords:
Brassica napus L.; purple leaf; anthocyanins; orthogonal experiment
PACS:
-
DOI:
10.11937/bfyy.201521032
Abstract:
Taking purple leaf Brassica napus as the research materials，the ortho-design experiment was carried out to study the influence of extraction time，extraction temperature，extraction agent and solid-liquid ratio on purple leaf Brassica napus anthocyanins extraction yield，and to establish the best extraction process.The results showed that factors which affected the anthocyanins extraction yield of purple leaf Brassica napus were extraction time>solid-liquid ratio>extracting temperature>extracting agent type，and the optimum technology parameters were follows,the extracting temperature was 60℃；extracting agent was 3% hydrochloric acid+90% ethanol；solid-liquid ratio was 1∶15 g/mL and the extracting time was 5 h.

References:

 

[1]KONG J M，CHIA L S，GOH N K，et al.Analysis and biological activities of anthocyanins[J].Phytochemistry，2003，64(5):923-933.

[2]KOWCLCZYK E，KRZESINSKI P，KURA M，et al.Anthocyanins in medicine [J].Pol J Pharmacol，2003，55(5):699-702.

[3]郭红辉，卫晓怡，凌文华.花色苷代谢的研究进展[J].食品研究与开发，2011，32（5）：163-166.

[4]PARKER J.Relationships among cold hardiness，water soluble protein，anthocyanins and free sugar in Hedera heliz L [J].Plant Physiol，1962，37：809-813.

[5]孙明霞，王宝增，范海等.叶片中的花色素苷及其对植物适应环境的意义[J].植物生理学通讯，2003，39：675-688.

[6]BAHLER B D，STEFFEN K L，ORROLEK M D.Morphological and biochemical comparison of a purple-leafed and a green-leafed pepper cultiva[J].Hort Sci，1991(26):73-76.

[7]STEYN W J，WAND S J E，HOLCROFT D M et al.Anthocyanins in vegetative tissues：a proposed unified function in photoprotection[J].New Phytologist，2002，155(3):349-361.

[8]陈勇，赵春芬，孙清，等.紫苏色素稳定性的初步研究[J].中国食品添加剂，1997(3)：4-8.

[9]潘小红，李金林，刘成梅,等.金属离子和食品添加剂对紫甘薯花色苷稳定性的影响[J].食品工业科技，2007，28（11）：35-38.

[10]CORTES G A，SALINAS M Y，MARTIN E S.Stability of anthocyanins of blue maize after nixtarnlization of separated pericarp-germ tip cap and endosperm fractions[J].Journal of Cereal Science，2006，43(1)：57-62.

[11]TIBOR FULEKI，FRANCIS F J.Quantitative methods for anthocyanins，1，extraction and determination of total anthocyanin in cranberries[J].J of Food Sci，1968，33：72.

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Last Update: 2015-12-28