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Isolation and Identification of Antagonistic Bacteria Against Postharvest Blue Mold Decay of Apple and Its Mutation by Low Energy N+Implantation(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2015年21
Page:
116-120
Research Field:
Publishing date:

Info

Title:
Isolation and Identification of Antagonistic Bacteria Against Postharvest Blue Mold Decay of Apple and Its Mutation by Low Energy N+Implantation
Author(s):
FU Ruimin1; 2; XING Wenhui1; GU Yanan2; XUE Tingting2; GUO Yanzhao2; CHEN Wuling2
（1.Department of Life Science，Henan Institute of Education,Zhengzhou，Henan 450046；2.College of Life Science，Northwest University,Xi’an，Shaanxi 710069）
Keywords:
apple; postharvest; blue mold decay; Bacillus amyloliquefaciens; low energy N+ implantation
PACS:
-
DOI:
10.11937/bfyy.201521030
Abstract:
Taking an antagonistic strain BA-16 which could inhibit Penicillium expansum isolated from apple surface as metarial，phenotypic，physiological，biochemical and phylogenetic (16SrRNA) charactors were studied.The strain BA-16 was identified as Bacillus amyloliquefaciens. In order to enhance its antagonistic capability，the mutation of BA-16 was carried out by using low energy N+ implantation.After the mutation，the mutant BA-16-8，which showed the strongest antagonist capability and stable hereditary stability was selected out.Aiming at estimating the increasing biological control effects of apple blue mold decay by mutant BA-16-8，the inhibition effects of bacterial broth and cell-free fermentation broth of mutant and wild-type strain were studied by biological control trial on fruit.The results showed that comparing with its wild type,the mutant displayed significantly higher effect on controlling apple blue mold disease.Therefore,the mutant BA-16-8 will be a potential biological control agent against apple blue mold decay.

References:

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Last Update: 2015-12-28