|Table of Contents|

Effect of Natural Additives on Protocorm Proliferation，Differentiation and Rooting of Dendrobium officinale(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2015年21
Page:
107-110
Research Field:
Publishing date:

Info

Title:
Effect of Natural Additives on Protocorm Proliferation，Differentiation and Rooting of Dendrobium officinale
Author(s):
FANG Zhongming1; 2; BAI Genxiang1; ZENG Qisen1; ZOU Min1; ZENG Songjun3
(1.School of Life Science and Technology，Wuhan Bioengineering Institute，Wuhan，Hubei 430415；2.College of Life Science and Technology，Huazhong Agricultural University，Wuhan，Hubei 430070；3.South China Botanical Garden，Chinese Academy of Sciences，Guangzhou，Guangdong 510650)
Keywords:
:Dendrobium officinale; tissue culture; natural additives
PACS:
-
DOI:
10.11937/bfyy.201521028
Abstract:
Taking Yunnan Ruanjiao D.officinale as metarials，the effect of natural additives on protocorms proliferation，differentiation and rooting were studied.The results showed that high concentrations of banana juice could inhibit the proliferation of protocorm of D.officinale but banana juice was favorable to proliferation.The best protocorm proliferation medium of D.officinale was MS+6-BA 1 mg/L+NAA 1 mg/L+sucrose 20 g/L+agar 8 g/L+activated carbon 2 g/L+potato juice 200 g/L，with multiplication rate 6.8 after 60 days，and differentiation rate reached to 97% after 30 days.When adding banana，or potato juice in MS，it had an obvious effect on rooting.The optimal natural additives for rooting was potato juice 100 g/L.

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Last Update: 2015-12-28