|Table of Contents|

Study on Mutagenic Effects of Ethyl Methane Sulfonate on Impatiens balsamina(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2015年21
Page:
83-89
Research Field:
Publishing date:

Info

Title:
Study on Mutagenic Effects of Ethyl Methane Sulfonate on Impatiens balsamina
Author(s):
ZHANG Yongfu1; 2; LU Guizhi1; XIE Yingmei1; HUANG Heping1; 2; YIN Lixin1; 2; LIU Jiani1; 2
(1.School of Agriculture，Kunming University，Kunming，Yunnan 650214；2.Engineering Research Center for Urban Modern Agriculture of High Education in Yunnan Province，Kunming，Yunnan 650214)
Keywords:
ethyl methyl sulfonate; Impatiens balsamina; mutation; flower type; flower color
PACS:
-
DOI:
10.11937/bfyy.201521023
Abstract:
Light red Impatiens balsamina landraces was chosen as test materials to study mutagenic effects of the combination treatment of ethyl methane sulfonate (EMS) with different concentrations (0.5，1.0，2.0，4.0 mg/mL) and different times (1，2，3 days) on Impatiens balsamina，in order to determine the best dosage in Impatiens balsamina induced mutation breeding，and screened the high ornamental value of the mutation strain.The results showed that Impatiens balsamina seed germination rate，root length，coleoptile length，radicle emergence rate，number，plant height，stem diameter，leaf length，leaf width and leaf shape index were controlled by chemical concentration and treatment time，when the concentration of 40 mg/mL treatment time was 2 days and 3 days，the germination rate was 0.Semi-lethal dose was 2 mg/mL for 2 days treatment，but this dose not obtained more excellent mutant，and 0.5-1.0 mg/mL treated for 2-3 days was able to obtain more excellent mutants with high ornamental value.The mutant types include terminal flower，a handle to spend fascicled，double flower，flower，flower stalk，block hinge fitting an extremely shortened and no trunk type mutant.Color also appeared deep red，pale pink，amaranth，pale pink，white powder，pale purplish red，purple，pink，white，modena，lilac，the purple powder，red and red and white mosaic color more than 10 kinds of mutation types.In these types of mutations，the individual plant of ornamental high value were purple red double flower，the crimson white chimeric double flower，purple double flower，the pale pink peony type double flower，red camellia type double flower，red and white roses type chimeric double flower，pink papilionaceous double flower，a handle to spend clustered type，pedicel in extremely short double chimeric flowers a total of 9.In addition，after processing of Impatiens balsamina petals pigment substances and nutrients were changed in different degree.The generational screening could be expected to obtain new varieties in the progeny of commercial production，or for Impatiens balsamina breeding to provide ideal materials.

References:

 

[1]汤泽生,杨军,刘平.卫星搭载对凤仙花减数分裂和小孢子的影响[J].核农学报,2005,19(1):33-36.

[2]陈艺林.中国植物志(第47卷第2分册)[M].北京:科学出版社,2001.

[3]南京中医药大学.中药大辞典[M].上海:上海科学技术出版社,2005.

[4]王峰,徐飚,杨正林,等.EMS诱变水稻矮生资源的鉴定评价[J].核农学报,2011,25(2):197-201.

[5]陈洋,高兰英,邵艳军,等.EMS诱导小麦易位系YW642突变体的鉴定与分子标记分析[J].核农学报,2011,25(4):617-621.

[6]张明义,王翔,张彦芹,等.玉米EMS诱变材料的抗旱性筛选[J].山西农业科学,2011,39(2):99-102.

[7]韩锁义,杨玛丽,陈远东,等.大豆“南农94-16”突变体库的构建及部分性状分析[J].核农学报,2008,22(2):131-135.

[8]王传堂,王秀贞,唐月异,等.EMS直接注入花生花器创制高产突变体[J].核农学报,2010,24(2):239-242.

[9]李浩杰,蒲晓斌,张锦芳,等.甘蓝型油菜EMS诱变后代农艺性状观察及分子检测[J].核农学报,2012,26(2):245-249.

[10]张宏军,肖钢,谭太龙,等.EMS处理甘蓝型油菜(Brassica napus)获得高油酸材料[J].中国农业科学,2008,41(12):4016-4022.

[11]蔡海燕,温立柱,郑成淑,等.EMS诱发菊花突变类型及重要性状的分子鉴定[J].山东农业大学学报,2013,44(2):171-175.

[12]马晓菲,唐玉倩,于元杰.高皂苷轮叶党参EMS诱变突变体研究[J].西北植物学报,2014,34(6):1125-1130.

[13]杨军,李劲涛,汤泽生,等.航天诱导凤仙花SP4代三种不同花色突变系比较[J].云南植物研究,2008,30(2):221-226.

[14]赵曼祯.“离子注入”诱变中国凤仙花[J].中国花卉园艺,2010(6):20-22.

[15]WROLSTAD R E,CULBERTSON J D,CORNWELL C J.Detection of adulteration in blackberry juice concentrates and wines[J].Journal Association Official Analytical Chemists,1982,65:1417-1423.

[16]SINGLETON V L,ROSSI J A.Colorimetry of total phenolics with phosphomolybdic-phosphotungstic acid reagents[J].American Journal of Enology Viticulture,1965,16:144-158.

[17]游新侠,仇农学.超声波辅助提取荆芥叶中总黄酮的方法研究[J].西北农业学报,2006,26(1):13-15.

[18]邹琦.植物生理学实验指导[M].北京:中国农业出版社,2000.

[19]安学丽,蔡一林,王久光,等.化学诱变及其在农作物育种上的应用[J].核农学报,2003,17(3):239-242.

[20]LATADO R R,ADAMES A H.In vitro mutation of chrysanthemum with EMS in immature floral pedicels[J].Plant Cell Tissue and Organ Culture,2004,77(1):103-106.

[21]崔霞,梁燕,王玲慧.不同浓度化学诱变剂甲基磺酸乙酯对番茄种子发芽的影响[J].北方园艺,2013(14):28-30.

[22]赵天祥,孔秀英,周荣华,等.EMS诱变六倍体小麦偃展4110的形态突变体鉴定与分析[J].中国农业科学,2009,42(3):755-764.

[23]OLOF O,AAKASH C,PER S.Developlement and characterization of an oat TILLING-population and identification of mutations in lignin and β-glucan biosynthesis genes[J].BMC Plant Biology,2010,10:86.

[24]张娜,杨希文,任长忠,等.白燕2号EMS突变体的形态鉴定与遗传变异分析[J].麦类作物学报,2011,31(3):421-426.

[25]REDDY T V,DWIYEDI S,SHARMA N K.Development of TILLING by sequencing platform towards enhanced leaf yield in tobacco[J].Industrial Crops Products,2012,40:324-335.

[26]LIU Z,ZHANG K,LIN J F.Breeding cold tolerance strain by chemical mutagenesis in Volvariella volvacea[J].Scientia Horticulturae,2011,130(1):18-24.

[27]黄永娟,张凤启,杨甜甜,等.EMS诱变甘蓝型油菜获得高油酸突变体[J].分子植物育种,2011,9(5):611-616.

[28]曲高平,孙妍妍,庞红喜,等.甘蓝型油菜EMS 突变体库构建及抗除草剂突变体筛选[J].中国油料作物学报,2014,36(1):25-31.

Memo

Memo:
-
Last Update: 2015-12-23