|Table of Contents|

Rapid Detection of Blueberry Shock Virus by RT-PCR(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2015年20
Page:
98-101
Research Field:
Publishing date:

Info

Title:
Rapid Detection of Blueberry Shock Virus by RT-PCR
Author(s):
XIE Lixue1CAI Wei2ZHENG Shan1ZHANG Lijie1ZHANG Xiaoyan1LI Tao1
(1.Fruit Research Institute,Fujian Academy of Agricultural Sciences,Fuzhou,Fujian 350013;2.Fuqing Entry-Exit Inspection and Quarantine Bureau,Fuqing,Fujian 350001)
Keywords:
blueberry shock virusRT-PCRrapid detection
PACS:
-
DOI:
10.11937/bfyy.201520024
Abstract:
RT-PCR method was established for rapid detection of Blueberry shock virus (BlShV).A set of specific primers were designed based on the published nucleotide sequence of BlShV coat protein gene.The viral RNA extracted from infected blueberry sample was used as template.Twenty suspected blueberry samples were detected by using the established method.The results showed that RT-PCR method had good specificity and high sensitivity.The method could amplify target fragment from BlShV-infected sample,while not from other blueberry virus infected sample and healthy sample.The sensitivity of RT-PCR method was 10-4 RNA.The detection result of suspected blueberry samples was consistent with ELISA,which indicated the RT-PCR method can be actually used for detection of BlShV.

References:

 

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Last Update: 2015-11-19