|Table of Contents|

Preliminary Expression Analysis of Seedling Under Salt-tolerance in Beach Plum Using cDNA-AFLP(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2015年20
Page:
81-85
Research Field:
Publishing date:

Info

Title:
Preliminary Expression Analysis of Seedling Under Salt-tolerance in Beach Plum Using cDNA-AFLP
Author(s):
ZHANG ChunhongHU ShuyingWU WenlongLI Weilin
(Institute of Botany,Jiangsu Province and Chinese Academy of Sciences,Nanjing,Jiangsu 210014)
Keywords:
beach plumcDNA-AFLPsalt-tolerancegene expression analysis
PACS:
-
DOI:
10.11937/bfyy.201520020
Abstract:
Using rooted ‘Beach plum No.5′ seedlings as experimental material,differential gene expression analysis was performed under salt treatment of 200 mmol/L NaCl by using cDNA-AFLP (Amplified fragment length polymorphism).Expression patterns under salt-tolerance and in different tissues of the candidate genes were then revealed by semi-quantitative RT-PCR validation.The results showed that a total of 4 263 distinguishable bands were obtained by amplification through 256 pairs of selective primer combinations,and 47 bands were obtained with significant differences.After cloning and sequencing of 24 bands,sequence analysis of them showed that main functions of the 14 differential sequence segments comprised of metabolism,transcription factors,stress-related proteins,etc.Two fragments related to plant resistance were obtained and had the 92% homology with butterfly grass asparagine synthetase,and 81% homology with wild strawberry sulfate transporter gene respectively.The differential band homologous to the wild strawberry sulfate transporter gene was obtained and the expression level reached maximum under 3 hours treatment followed by gradual decrease with the increased processing time.Tissue expression analysis indicated that it had the highest level in roots indicating it mainly played a vital role in roots.

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Last Update: 2015-11-19