Effect of Different Concentrations of Colchicine on the Plyploid Breeding of Paris polyphylla(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Title:: Effect of Different Concentrations of Colchicine on the Plyploid Breeding of Paris polyphylla

Author(s):: YANG Yali; LIU Jia; ZHANG Peng; SONG Fajun; YU Xiaodong; WANG Chuntai; YANG Yali，LIU Jia，ZHANG Peng，SONG Fajun，YU Xiaodong，WANG Chuntai

Abstract:: In order to investigate the influence of different concentrations of colchicine on the plyploid breeding of Paris polyphylla, P.polyphylla seeds as experimental materials were treated by colchicine with the concentration of 0.025%，0.050%，0.100%，0.150% and 0.200% for different time of 12，24，48，60 hours，respectively.The results showed that the root tip swelling of polyphylla seeds treated with different colchicine concentrations for different times were more than the control seeds untreated with colchicine，and the highest swelling rate was 51.69% under the treatment of 0.05% colchicine for 12 hours.The radicles of colchicine-treated P.polyphylla seeds and the plants originated from colchicine-treated seeds were shorter and stronger than the control untreated with colchicine.The detection results of flow cytometry showed that the DNA fluorescence intensity of the radicle cells of the plants originated from colchicine-treated seeds was about 1.88 folds of the control non-treated with colchicine，indicating that the chromosome of the plants originated from colchicine-treated seeds were partially doubled.These results provided references for the development of polyploid breeding of P.polyphylla.

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Last Update: 2015-11-18